BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 30386

Title: Two-State 14-mer UUCG Tetraloop calculated from Exact NOEs (State one: Conformers 1-5, State Two: Conformers 6-10)

Authors: Nichols, P.; Henen, M.; Born, A.; Strotz, D.; Guntert, P.; Vogeli, B.

Citation: Nichols, P.; Henen, M.; Born, A.; Strotz, D.; Guntert, P.; Vogeli, B.. "High-Resolution 14-mer UUCG Tetraloop from Exact NOE Distances Alone"  . ., .-..

Assembly members:
entity_1, polymer, 14 residues, 4454.683 Da.

Natural source:   Common Name: not available   Taxonomy ID: 32630   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: GGCACUUCGGUGCC

Data typeCount
1H chemical shifts200

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 14 residues - 4454.683 Da.

1   GGCACUUCGG
2   UGCC

Samples:

sample_1: KHPO4 20 mM; EDTA 0.4 mM; 14-mer UUCG Tetraloop 1 mM

sample_2: KHPO4 20 mM; EDTA 0.4 mM; 14-mer UUCG Tetraloop 1.7 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D WaterGate [1H-1H] NOESY buildup seriessample_1isotropicsample_conditions_1
2D PreSat [1H-1H] NOESY buildup seriessample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNMR, CCPN - peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - data analysis, refinement, structure calculation

NMR spectrometers:

  • Varian Varian Direct Drive 900 MHz

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