BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 30374

Title: HSPB5 alpha-crystallin domain mutant R120G-ACD

Authors: Rajagopal, P.; Klevit, R.

Citation: Rajagopal, P.; Klevit, R.. "HSPB5 alpha-crystallin domain mutant R120G-ACD"  . ., .-..

Assembly members:
entity_1, polymer, 89 residues, 10099.370 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: GLSEMRLEKDRFSVNLDVKH FSPEELKVKVLGDVIEVHGK HEERQDEHGFISREFHGKYR IPADVDPLTITSSLSSDGVL TVNGPRKQV

Data typeCount
13C chemical shifts375
15N chemical shifts98
1H chemical shifts492

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_1, 11
2entity_1, 21

Entities:

Entity 1, entity_1, 1 89 residues - 10099.370 Da.

1   GLYLEUSERGLUMETARGLEUGLULYSASP
2   ARGPHESERVALASNLEUASPVALLYSHIS
3   PHESERPROGLUGLULEULYSVALLYSVAL
4   LEUGLYASPVALILEGLUVALHISGLYLYS
5   HISGLUGLUARGGLNASPGLUHISGLYPHE
6   ILESERARGGLUPHEHISGLYLYSTYRARG
7   ILEPROALAASPVALASPPROLEUTHRILE
8   THRSERSERLEUSERSERASPGLYVALLEU
9   THRVALASNGLYPROARGLYSGLNVAL

Samples:

sample_1: HSPB5 mutant R120G-ACD, [U-13C; U-15N], 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; EDTA 100 uM; PMSF 1 mM

sample_2: HSPB5 mutant R120G-ACD, [U-13C; U-15N; 50%-2H], 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; EDTA 100 uM; PMSF 1 mM

sample_3: HSPB5 mutant R120G-ACD, [U-13C; U-15N; U-2H], 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; EDTA 100 uM; PMSF 1 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D NOESYsample_1isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCAsample_3isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_3isotropicsample_conditions_1
3D HN(COCA)CBsample_3isotropicsample_conditions_1
3D HN(CO)CAsample_3isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

CSRosetta, David Baker - structure calculation

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker AvanceII 600 MHz
  • Varian UNITYPLUS 800 MHz
  • Bruker AvanceIII 500 MHz
  • Varian UNITYPLUS 900 MHz
  • Varian UNITYPLUS 600 MHz

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