BMRB Entry 30315

Title:
AsK132958: A minimal homologue of ShK identified in the transcriptome of Anemonia sulcata
Deposition date:
2017-07-02
Original release date:
2017-10-19
Authors:
Krishnarjuna, B.; MacRaild, C.; Norton, R.
Citation:

Citation: Krishnarjuna, Bankala; MacRaild, Christopher; Sunanda, Punnepalli; Morales, Rodrigo; Peigneur, Steve; Macrander, Jason; Yu, Heidi; Daly, Marymegan; Raghothama, Srinivasarao; Dhawan, Vikas; Chauhan, Satendra; Tytgat, Jan; Pennington, Michael; Norton, Raymond. "Structure, folding and stability of a minimal homologue from Anemonia sulcata of the sea anemone potassium channel blocker ShK"  Peptides 99, 169-178 (2017).
PubMed: 28993277

Assembly members:

Assembly members:
ShK homolog AsK132958, polymer, 29 residues, 3134.659 Da.

Natural source:

Natural source:   Common Name: snake-locks sea anemone   Taxonomy ID: 6108   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Anemonia Sulcata

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ShK homolog AsK132958: CENTISGCSRADCLLTHRKQ GCQKTCGLC

Data sets:
Data typeCount
15N chemical shifts33
1H chemical shifts187

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 29 residues - 3134.659 Da.

1   CYSGLUASNTHRILESERGLYCYSSERARG
2   ALAASPCYSLEULEUTHRHISARGLYSGLN
3   GLYCYSGLNLYSTHRCYSGLYLEUCYS

Samples:

sample_1: AsK132958 1 mM

sample_conditions_1: ionic strength: 0 Not defined; pH: 4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
1D 1Hsample_1isotropicsample_conditions_1

Software:

Analysis, CCPN - chemical shift assignment, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

XPLOR-NIH, G. Marius Clore, Guillermo Bermejo, John Kuszewski, Charles D. Schwieters, and Nico Tjandra - refinement

NMR spectrometers:

  • Bruker AvanceIII 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks