BMRB Entry 30283

Title:
Structure and dynamics of RNA repeat expansions that cause Huntington's Disease and myotonic dystrophy type 1
Deposition date:
2017-04-12
Original release date:
2017-06-23
Authors:
Chen, J.; VanEtten, D.; Fountain, M.; Yildirim, I.; Disney, M.
Citation:

Citation: Chen, J.; VanEtten, D.; Fountain, M.; Yildirim, I.; Disney, M.. "Structure and dynamics of RNA repeat expansions that cause Huntington's Disease and myotonic dystrophy type 1"  Biochemistry 56, 3463-3474 (2017).
PubMed: 28617590

Assembly members:

Assembly members:
entity_1, polymer, 15 residues, 4760.849 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GACCUGCUGCUGGUC

Data typeCount
1H chemical shifts94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_1, 11
2entity_1, 21

Entities:

Entity 1, entity_1, 1 15 residues - 4760.849 Da.

1   GACCUGCUGC
2   UGGUC

Samples:

sample_1: EDTA 0.25 mM; RNA (5'-R(*GP*AP*CP*CP*UP*GP*CP*UP*GP*CP*UP*GP*GP*UP*C)-3'), NA Adenosine, Cytidine,Guanosine, Uridine, 0.5 mM; potassium phosphate 5.0 mM

sample_2: EDTA 0.25 nM; RNA (5'-R(*GP*AP*CP*CP*UP*GP*CP*UP*GP*CP*UP*GP*GP*UP*C)-3'), NA Adenosine, Cytidine,Guanosine, Uridine, 0.5 uM; potassium phosphate 5.0 mM

sample_conditions_2: ionic strength: 0.0112 M; pH: 6; pressure: 1 atm; temperature: 276 K

sample_conditions_1: ionic strength: 0.0112 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_2

Software:

AMBER v16, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - data analysis, structure calculation

NMRPipe v8.9, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.0, Goddard - chemical shift assignment, peak picking

TOPSPIN v3.5, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance III HD UltraShield 700 MHz
  • Bruker Avance III HD Ascend 850 MHz