BMRB Entry 30237

Title:
NMR structure of monomeric human IRAK-M Death Domain R56D, Y61E mutant
Deposition date:
2017-01-20
Original release date:
2018-01-17
Authors:
Kwon, J.; Nicholson, L.
Citation:

Citation: Nechama, Morris; Kwon, Jeahoo; Wei, Shuo; Kyi, Adrian Tun; Welner, Robert; Ben-Dov, Iddo; Arredouani, Mohamed; Asara, John; Chen, Chun-Hau; Tsai, Cheng-Yu; Nelson, Kyle; Kobayashi, Koichi; Israel, Elliot; Zhou, Xiao Zhen; Nicholson, Linda; Lu, Kun Ping. "The IL-33-PIN1-IRAK-M axis is critical for type 2 immunity in IL-33-induced allergic airway inflammation."  Nat. Commun. 9, 1603-1603 (2018).
PubMed: 29686383

Assembly members:

Assembly members:
entity_1, polymer, 124 residues, 13467.164 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMAL-c2X

Data sets:
Data typeCount
13C chemical shifts483
15N chemical shifts131
1H chemical shifts742

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 124 residues - 13467.164 Da.

1   SERGLUPHEGLYSERMETALAGLYASNCYS
2   GLYALAARGGLYALALEUSERALAHISTHR
3   LEULEUPHEASPLEUPROPROALALEULEU
4   GLYGLULEUCYSALAVALLEUASPSERCYS
5   ASPGLYALALEUGLYTRPARGGLYLEUALA
6   GLUARGLEUSERSERSERTRPLEUASPVAL
7   ASPHISILEGLULYSGLUVALASPGLNGLY
8   LYSSERGLYTHRARGGLULEULEUTRPSER
9   TRPALAGLNLYSASNLYSTHRILEGLYASP
10   LEULEUGLNVALLEUGLNGLUMETGLYHIS
11   ARGARGALAILEHISLEUILETHRASNTYR
12   GLYALAVALLEUSERPROSERGLULYSSER
13   TYRGLNGLUGLY

Samples:

sample_1: IRAK-M Death Domain_R56D_Y61E, [U-99% 13C; U-99% 15N], 800 uM; TCEP 5 mM; TRIS 10 mM; sodium azide 5 mM; sodium chloride 20 mM

sample_conditions_1: ionic strength: 0.0229 M; pH: 6.67; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PINE v2.0, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

PONDEROSA, NMRFAM (University of Wisconsin-Madison), WoongHee Lee - structure calculation

SPARKY v3.115, Goddard - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks