BMRB Entry 30227

Title:
Zinc-Binding Structure of a Catalytic Amyloid from Solid-State NMR Spectroscopy
Deposition date:
2017-01-09
Original release date:
2017-05-25
Authors:
Lee, M.; Wang, T.; Makhlynets, O.; Wu, Y.; Polizzi, N.; Wu, H.; Gosavi, P.; Korendovych, I.; DeGrado, W.; Hong, M.
Citation:

Citation: Lee, Myungwoon; Wang, Tuo; Makhlynets, Olga; Wu, Yibing; Polizzi, Nicholas; Wu, Haifan; Gosavi, Pallavi; Stohr, Jan; Korendovych, Ivan; DeGrado, William; Hong, Mei. "Zinc-binding structure of a catalytic amyloid from solid-state NMR"  Proc. Natl. Acad. Sci. U. S. A. 114, 6191-6196 (2017).
PubMed: 28566494

Assembly members:

Assembly members:
entity_1, polymer, 7 residues, 861.042 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 32630   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: IHVHLQI

Data sets:
Data typeCount
13C chemical shifts69
15N chemical shifts18

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_1, 11
2entity_1, 21
3entity_1, 31
4entity_1, 41
5entity_1, 51
6entity_1, 61
7entity_1, 71
8entity_1, 81
9entity_1, 91
10entity_1, 101
11entity_1, 111
12entity_1, 121
13entity_ZN, 12
14entity_ZN, 22
15entity_ZN, 32
16entity_ZN, 42
17entity_ZN, 52
18entity_ZN, 62
19entity_ZN, 72
20entity_ZN, 82
21entity_ZN, 92
22entity_ZN, 102
23entity_ZN, 112
24entity_ZN, 122

Entities:

Entity 1, entity_1, 1 7 residues - 861.042 Da.

1   ILEHISVALHISLEUGLNILE

Entity 2, entity_ZN, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: IVL, [U-13C; U-15N], 0.4 g/L

sample_2: mixed sample, [U-13C], 0.4 g/L

sample_conditions_1: ionic strength: 100 mM; pH: 8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-13C correlation spectrasample_1isotropicsample_conditions_1
2D 15N-13C correlation spectrasample_1isotropicsample_conditions_1
REDORsample_1isotropicsample_conditions_1
2D 13C-13C correlation spectrasample_2isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

TALOS, Cornilescu, Delaglio and Bax - data analysis

TOPSPIN, Bruker Biospin - chemical shift assignment

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Bruker Bruker 800 MHz