BMRB Entry 30209

Title:
NMR structure of the precursor protein PawS1 comprising SFTI-1 and a seed storage albumin
Deposition date:
2016-12-14
Original release date:
2017-02-20
Authors:
Franke, B.; James, A.; Mobli, M.; Colgrave, M.; Mylne, J.; Rosengren, K.
Citation:

Citation: Franke, B.; James, A.; Mobli, M.; Colgrave, M.; Mylne, J.; Rosengren, K.. "NMR structure of the precursor protein PawS1 comprising SFTI-1 and a seed storage albumin"  to be published ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 116 residues, 13280.334 Da.

Natural source:

Natural source:   Common Name: Common sunflower   Taxonomy ID: 4232   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Helianthus annuus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts482
15N chemical shifts109
1H chemical shifts765

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 116 residues - 13280.334 Da.

1   GLYARGCYSTHRLYSSERILEPROPROILE
2   CYSPHEPROASPGLYLEUASPASNPROARG
3   GLYCYSGLNILEARGILEGLNGLNLEUASN
4   HISCYSGLNMETHISLEUTHRSERPHEASP
5   TYRLYSLEUARGMETALAVALGLUASNPRO
6   LYSGLNGLNGLNHISLEUSERLEUCYSCYS
7   ASNGLNLEUGLNGLUVALGLULYSGLNCYS
8   GLNCYSGLUALAILELYSGLNVALVALGLU
9   GLNALAGLNLYSGLNLEUGLNGLNGLYGLN
10   GLYGLYGLNGLNGLNVALGLNGLNMETVAL
11   LYSLYSALAGLNMETLEUPROASNGLNCYS
12   ASNLEUGLNCYSSERILE

Samples:

sample_1: Proalbumin PawS1, [U-13C; U-15N], 3.9 mg/mL

sample_conditions_1: ionic strength: 0 mM; pH: 4.62; pressure: 1 bar; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CC)(CO)NH-TOCSYsample_1isotropicsample_conditions_1
3D (H)CC(CO)NH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure calculation

CcpNMR, CCPN - peak picking

TOPSPIN v2.1, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks