BMRB Entry 30056

Title:
DIY G-Quadruplexes: Solution structure of d(GGTTTGGTTTTGGTTGG) in sodium
Deposition date:
2016-04-01
Original release date:
2017-04-06
Authors:
Dvorkin, S.; Webba da Silva, M.
Citation:

Citation: Dvorkin, Scarlett; Karsisiotis, Andreas; Webba da Silva, Mateus. "Encoding canonical DNA quadruplex structure."  Sci Adv 4, eaat3007-eaat3007 (2018).
PubMed: 30182059

Assembly members:

Assembly members:
DNA (5'-D(*GP*GP*TP*TP*TP*GP*GP*TP*TP*TP*TP*GP*GP*TP*TP*GP*G)-3'), polymer, 17 residues, 5326.425 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 32644   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DNA (5'-D(*GP*GP*TP*TP*TP*GP*GP*TP*TP*TP*TP*GP*GP*TP*TP*GP*G)-3'): GGTTTGGTTTTGGTTGG

Data sets:
Data typeCount
1H chemical shifts161

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 17 residues - 5326.425 Da.

1   DGDGDTDTDTDGDGDTDTDT
2   DTDGDGDTDTDGDG

Samples:

sample_1: DNA 2 ± 0.5 mM; NaPi 100 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 100 mM; pH: 6.8; pressure: 1 atm; temperature: 278.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

Felix, Accelrys Software Inc. - chemical shift assignment

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure calculation

NMR spectrometers:

  • Varian VXRS 500 MHz