BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30028

Title: GCN4p pH 4.4   PubMed: 28230348

Authors: Brady, M.; Kaplan, A.; Alexandrescu, A.

Citation: Kaplan, Anne; Brady, Megan; Maciejewski, Mark; Kammerer, Richard; Alexandrescu, Andrei. "Nuclear Magnetic Resonance Structures of GCN4p Are Largely Conserved When Ion Pairs Are Disrupted at Acidic pH but Show a Relaxation of the Coiled Coil Superhelix"  Biochemistry 56, 1604-1619 (2017).

Assembly members:
entity_1, polymer, 33 residues, 3835.406 Da.

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: GSMKQLEDKVEELLSKNYHL ENEVARLKKLVGE

Data sets:
Data typeCount
13C chemical shifts135
15N chemical shifts35
1H chemical shifts238

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_21

Entities:

Entity 1, entity_1 33 residues - 3835.406 Da.

1   GLYSERMETLYSGLNLEUGLUASPLYSVAL
2   GLUGLULEULEUSERLYSASNTYRHISLEU
3   GLUASNGLUVALALAARGLEULYSLYSLEU
4   VALGLYGLU

Samples:

sample_1: GCN4p, [U-99% 13C; U-99% 15N], 1.5 mM

sample_2: GCN4p, [U-99% 13C; U-99% 15N], 1.5 mM

sample_conditions_1: ionic strength: 20 mM; pH: 4.4; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 20 mM; pH: 4.6; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_2
2D 15N NOESYsample_1isotropicsample_conditions_1

Software:

ARIA, Linge, O'Donoghue and Nilges - refinement

CcpNMR, CCPN - chemical shift assignment

Felix, Accelrys Software Inc. - processing

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure calculation

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links: