BMRB Entry 28059

Title:
1H-15N backbone Heteronuclear NOE values for free and ssDNA complex forms of Human YB1 cold shock domain.
Deposition date:
2020-01-02
Original release date:
2020-01-17
Authors:
Fan, Jing-Song; Yang, Daiwen
Citation:

Citation: Zhang, Jingfeng; Fan, Jing-Song; Li, Shuangli; Yang, Yunhuang; Sun, Peng; Zhu, Qinjun; Wang, Jiannan; Jiang, Bin; Yang, Daiwen; Liu, Maili. "Structural basis of DNA binding to human YB-1 cold shock domain regulated by phosphorylation"  Nucleic Acids Res. 48, 9361-9371 (2020).
PubMed: 32710623

Assembly members:

Assembly members:
CSD, polymer, 117 residues, Formula weight is not available
ssDNA, polymer, 7 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet32a

Data typeCount
heteronuclear NOE values157

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CSD1
2ssDNA2

Entities:

Entity 1, CSD 117 residues - Formula weight is not available

1   METHISHISHISHISHISHISGLYGLYGLY
2   GLYGLYGLYGLYGLYSERSERALAALAPRO
3   ALAGLYGLYASPLYSLYSVALILEALATHR
4   LYSVALLEUGLYTHRVALLYSTRPPHEASN
5   VALARGASNGLYTYRGLYPHEILEASNARG
6   ASNASPTHRLYSGLUASPVALPHEVALHIS
7   GLNTHRALAILELYSLYSASNASNPROARG
8   LYSTYRLEUARGSERVALGLYASPGLYGLU
9   THRVALGLUPHEASPVALVALGLUGLYGLU
10   LYSGLYALAGLUALAALAASNVALTHRGLY
11   PROGLYGLYVALPROVALGLNGLYSERLYS
12   TYRALAALAASPARGASNHIS

Entity 2, ssDNA 7 residues - Formula weight is not available

1   DADADCDADCDCDT

Samples:

sample_1: CSD, [U-100% 15N], 1 mM; phosphate buffer 20 mM; NaCl 50 mM

sample_2: CSD, [U-100% 15N], 1 mM; phosphate buffer 20 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 20 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N heteronuckear NOEsample_1isotropicsample_conditions_1
1H-15N heteronuckear NOEsample_2isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz