BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 28046

Title: RalA.GMPPNP   PubMed: 31916136

Deposition date: 2019-11-25 Original release date: 2020-02-26

Authors: Shafiq, Arooj; Owen, Darerca

Citation: Shafiq, Arooj; Campbell, Louise; Owen, Darerca; Mott, Helen. "NMR resonance assignments for the active and inactive conformations of the small G protein RalA"  Biomol. NMR Assignments 14, 87-91 (2020).

Assembly members:
RalA, polymer, 184 residues, Formula weight is not available
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER, non-polymer, 522.196 Da.

Natural source:   Common Name: cotton-top tamarin   Taxonomy ID: 9490   Superkingdom: Eukaryota   Kingdom: metazoa   Genus/species: Saguinus Oedipus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
RalA: MAANKPKGQNSLALHKVIMV GSGGVGKSALTLQFMYDEFV EDYEPTKADSYRKKVVLDGE EVQIDILDTAGLEDYAAIRD NYFRSGEGFLCVFSITEMES FAATADFREQILRVKEDENV PFLLVGNKSDLEDKRQVSVE EAKNRADQWNVNYVETSAKT RANVDKVFFDLMREIRARKM EDSK

Data sets:
Data typeCount
13C chemical shifts583
15N chemical shifts177
1H chemical shifts1189

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RalA1
2PNP2

Entities:

Entity 1, RalA 184 residues - Formula weight is not available

1   METALAALAASNLYSPROLYSGLYGLNASN
2   SERLEUALALEUHISLYSVALILEMETVAL
3   GLYSERGLYGLYVALGLYLYSSERALALEU
4   THRLEUGLNPHEMETTYRASPGLUPHEVAL
5   GLUASPTYRGLUPROTHRLYSALAASPSER
6   TYRARGLYSLYSVALVALLEUASPGLYGLU
7   GLUVALGLNILEASPILELEUASPTHRALA
8   GLYLEUGLUASPTYRALAALAILEARGASP
9   ASNTYRPHEARGSERGLYGLUGLYPHELEU
10   CYSVALPHESERILETHRGLUMETGLUSER
11   PHEALAALATHRALAASPPHEARGGLUGLN
12   ILELEUARGVALLYSGLUASPGLUASNVAL
13   PROPHELEULEUVALGLYASNLYSSERASP
14   LEUGLUASPLYSARGGLNVALSERVALGLU
15   GLUALALYSASNARGALAASPGLNTRPASN
16   VALASNTYRVALGLUTHRSERALALYSTHR
17   ARGALAASNVALASPLYSVALPHEPHEASP
18   LEUMETARGGLUILEARGALAARGLYSMET
19   GLUASPSERLYS

Entity 2, PNP - C10 H17 N6 O13 P3 - 522.196 Da.

1   GNP

Samples:

sample_1: RalA, [U-15N], 0.26 ± 0.1 mM; GMPPNP 0.26 ± 0.1 mM; NaCl 150 mM

sample_2: RalA, [U-15N; U-13C], 0.45 ± 0.1 mM; GMPPNP 0.45 ± 0.1 mM; NaCl 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HCCH TOCSYsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1

Software:

CCPN v2.4.2, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 800 MHz