BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 28036

Title: Shaker-VSD(S2bot_mutant)

Deposition date: 2019-11-04 Original release date: 2020-01-10

Authors: Chen, Hongbo; Chanda, Baron; Henzler-Wildman, Katherine

Citation: Chen, Hongbo; Chanda, Baron; Henzler-Wildman, Katherine. "1H, 15N and 13C backbone chemical shift assignment of Shaker-VSD(S2bot_mutant)"  Not known ., .-..

Assembly members:
Shaker-VSD(S2bot_mutant), polymer, 185 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Shaker-VSD(S2bot_mutant): GGGGFEYPESSQAARVVAII SVFVILLSIVIFCLETLPEF KHYKVFNTTTNGTKIEEDEV PDITDPFFLIETLCIIWFTF ELTVRFLACPNKLNFCRDVM NVIDIIAIIPYFITLATVVA EEEDTLNLPKAPVSPQDKSS NQAMSLAILRVIRLVRSSRI FKLSRHSKGLQILGRTLKAS MRELG

Data sets:
Data typeCount
13C chemical shifts510
15N chemical shifts155
1H chemical shifts155

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Shaker-VSD(S2bot_mutant)1

Entities:

Entity 1, Shaker-VSD(S2bot_mutant) 185 residues - Formula weight is not available

1   GLYGLYGLYGLYPHEGLUTYRPROGLUSER
2   SERGLNALAALAARGVALVALALAILEILE
3   SERVALPHEVALILELEULEUSERILEVAL
4   ILEPHECYSLEUGLUTHRLEUPROGLUPHE
5   LYSHISTYRLYSVALPHEASNTHRTHRTHR
6   ASNGLYTHRLYSILEGLUGLUASPGLUVAL
7   PROASPILETHRASPPROPHEPHELEUILE
8   GLUTHRLEUCYSILEILETRPPHETHRPHE
9   GLULEUTHRVALARGPHELEUALACYSPRO
10   ASNLYSLEUASNPHECYSARGASPVALMET
11   ASNVALILEASPILEILEALAILEILEPRO
12   TYRPHEILETHRLEUALATHRVALVALALA
13   GLUGLUGLUASPTHRLEUASNLEUPROLYS
14   ALAPROVALSERPROGLNASPLYSSERSER
15   ASNGLNALAMETSERLEUALAILELEUARG
16   VALILEARGLEUVALARGSERSERARGILE
17   PHELYSLEUSERARGHISSERLYSGLYLEU
18   GLNILELEUGLYARGTHRLEULYSALASER
19   METARGGLULEUGLY

Samples:

15N-13C-D_S2bot: H2O 90%; D2O 10%; Shaker-VSD(S2bot_mutant), [U-13C; U-15N; U-2H], 0.35 mM; potassium chloride 50 mM; MOPS 100 mM; LPPG 40 mM; TCEP 4 mM

sample_conditions_1: pH: 6.7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15N-13C-D_S2botisotropicsample_conditions_1
3D HNCO15N-13C-D_S2botisotropicsample_conditions_1
3D HNCA15N-13C-D_S2botisotropicsample_conditions_1
3D HNCACB15N-13C-D_S2botisotropicsample_conditions_1
3D HN(CO)CA15N-13C-D_S2botisotropicsample_conditions_1
3D HCACO15N-13C-D_S2botisotropicsample_conditions_1

Software:

CCPNmr_Analysis, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 750 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts