BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 28028

Title: 1H, 13C, 15N backbone and side-chain assignment of the native form of UbcH7 (UBE2L3)   PubMed: 31792831

Deposition date: 2019-10-09 Original release date: 2020-01-30

Authors: Marousis, Konstantinos; Seliami, Anastasia; Birkou, Maria; Spyroulias, Georgios

Citation: Marousis, Konstantinos; Birkou, Maria; Asimakopoulou, Antonia; Spyroulias, Georgios. "1H, 13C, 15N backbone and side-chain resonance assignment of the native form of UbcH7 (UBE2L3) through solution NMR spectroscopy"  Biomol. NMR Assignments ., .-. (2019).

Assembly members:
UbcH7, polymer, 154 residues, 17861.58 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
UbcH7: MAASRRLMKELEEIRKCGMK NFRNIQVDEANLLTWQGLIV PDNPPYDKGAFRIEINFPAE YPFKPPKITFKTKIYHPNID EKGQVCLPVISAENWKPATK TDQVIQSLIALVNDPQPEHP LRADLAEEYSKDRKKFCKNA EEFTKKYGEKRPVD

Data sets:
Data typeCount
13C chemical shifts684
15N chemical shifts157
1H chemical shifts1116

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UbcH71

Entities:

Entity 1, UbcH7 154 residues - 17861.58 Da.

1   METALAALASERARGARGLEUMETLYSGLU
2   LEUGLUGLUILEARGLYSCYSGLYMETLYS
3   ASNPHEARGASNILEGLNVALASPGLUALA
4   ASNLEULEUTHRTRPGLNGLYLEUILEVAL
5   PROASPASNPROPROTYRASPLYSGLYALA
6   PHEARGILEGLUILEASNPHEPROALAGLU
7   TYRPROPHELYSPROPROLYSILETHRPHE
8   LYSTHRLYSILETYRHISPROASNILEASP
9   GLULYSGLYGLNVALCYSLEUPROVALILE
10   SERALAGLUASNTRPLYSPROALATHRLYS
11   THRASPGLNVALILEGLNSERLEUILEALA
12   LEUVALASNASPPROGLNPROGLUHISPRO
13   LEUARGALAASPLEUALAGLUGLUTYRSER
14   LYSASPARGLYSLYSPHECYSLYSASNALA
15   GLUGLUPHETHRLYSLYSTYRGLYGLULYS
16   ARGPROVALASP

Samples:

sample_15N: UbcH7, [U-99% 15N], 1.0 mM; K2HPO4/KH2PO4 50.0 mM

sample_15N-13C: UbcH7, [U-98% 13C; U-98% 15N], 1.0 mM; K2HPO4/KH2PO4 50.0 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_15Nisotropicsample_conditions_1
2D 1H-15N HSQCsample_15Nisotropicsample_conditions_1
3D HNHAsample_15Nisotropicsample_conditions_1
2D 1H-13C HSQCsample_15N-13Cisotropicsample_conditions_1
3D HNCOsample_15N-13Cisotropicsample_conditions_1
3D HNCAsample_15N-13Cisotropicsample_conditions_1
3D HNCACBsample_15N-13Cisotropicsample_conditions_1
3D HN(CO)CAsample_15N-13Cisotropicsample_conditions_1
3D HN(CA)COsample_15N-13Cisotropicsample_conditions_1
3D CBCA(CO)NHsample_15N-13Cisotropicsample_conditions_1
3D HBHA(CO)NHsample_15N-13Cisotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_15N-13Cisotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_15N-13Cisotropicsample_conditions_1
3D HCCH-TOCSYsample_15N-13Cisotropicsample_conditions_1

Software:

CARA v1.5.5, Keller and Wuthrich - chemical shift assignment

TOPSPIN v3.5 PL5, Bruker Biospin - processing

NMR spectrometers:

  • Bruker Avance III HD 700 MHz

Related Database Links:

UNP P68036

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts