BMRB Entry 27985

Name: Canine CXCL8
Published: 2021-07-21

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27985

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Canine CXCL8

Deposition date:

2019-07-24

Original release date:

2021-07-21

Authors:

Gangele, Krishnakant; Gulati, Khushboo; Kumar, Dinesh; Poluri, Krishna Mohan

Citation:

Citation: Gangele, Krishnakant; Gulati, Khushboo; Joshi, Nidhi; Kumar, Dinesh; Poluri, Krishna Mohan. "Molecular insights into the differential structure-dynamics-stability features of interleukin-8 orthologs: Implications to functional specificity" Int. J. Biol. Macromol. 164, 3221-3234 (2020).
PubMed: 32853623

Assembly members:

Assembly members:
Canine_CXCL8, polymer, 74 residues, 8578 Da.

Natural source:

Natural source: Common Name: dog Taxonomy ID: 9615 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Canis familiaris

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Canine_CXCL8: VSSELRCQCIKTHSTPFHPK YIKELRVIDSGPHCENSEII VKLFNGNEVCLDPKEKWVQK VVQIFLKKAEKQDP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	197
15N chemical shifts	68
1H chemical shifts	136

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Canine CXCL8	1

Entities:

Entity 1, Canine CXCL8 74 residues - 8578 Da.

Full length canine CXCL8 protein.

1

VAL

SER

GLU

LEU

ARG

CYS

GLN

CYS

ILE

2

LYS

THR

HIS

SER

THR

PRO

PHE

HIS

PRO

LYS

3

TYR

ILE

LYS

GLU

LEU

ARG

VAL

ILE

ASP

SER

4

GLY

PRO

HIS

CYS

GLU

ASN

SER

GLU

ILE

5

VAL

LYS

LEU

PHE

ASN

GLY

ASN

GLU

VAL

CYS

6

LEU

ASP

PRO

LYS

GLU

LYS

TRP

VAL

GLN

LYS

7

VAL

GLN

ILE

PHE

LEU

LYS

ALA

GLU

8

LYS

GLN

ASP

PRO

Samples:

sample_1: Canine CXCL8, [U-99% 13C; U-99% 15N], 1 mM; D2O, [U-100% 2H], 10%; H2O 90%; potassium phosphate 50 mM; sodium azide 0.05%

sample_conditions_1: ionic strength: 0.05 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.9.1, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 500 MHz