BMRB Entry 27969

Title:
Backbone amide and MILV methyl chemical shift assignments of mouse Interleukin-2
Deposition date:
2019-07-09
Original release date:
2020-04-03
Authors:
De Paula, Viviane; Sgourakis, Nikolaos
Citation:

Citation: De Paula, Viviane; Jude, Kevin; Nerli, Santrupti; Glassman, Caleb; Garcia, K Christopher; Sgourakis, Nikolaos. "Interleukin-2 druggability is modulated by global conformational transitions controlled by a helical capping switch"  Proc. Natl. Acad. Sci. U.S.A. 117, 7183-7192 (2020).
PubMed: 32184322

Assembly members:

Assembly members:
IL-2, polymer, 160 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMAL

Data sets:
Data typeCount
13C chemical shifts330
15N chemical shifts84
1H chemical shifts268

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IL-21

Entities:

Entity 1, IL-2 160 residues - Formula weight is not available

1   ALAPROTHRSERSERSERTHRSERSERSER
2   THRALAGLUALAGLNGLNGLNGLNGLNGLN
3   GLNGLNGLNGLNGLNGLNHISLEUGLUGLN
4   LEULEUMETASPLEUGLNGLULEULEUSER
5   ARGMETGLUASNTYRARGASNLEULYSLEU
6   PROARGMETLEUTHRPHELYSPHETYRLEU
7   PROLYSGLNALATHRGLULEULYSASPLEU
8   GLNCYSLEUGLUASPGLULEUGLYPROLEU
9   ARGHISVALLEUASPLEUTHRGLNSERLYS
10   SERPHEGLNLEUGLUASPALAGLUASNPHE
11   ILESERASNILEARGVALTHRVALVALLYS
12   LEULYSGLYSERASPASNTHRPHEGLUCYS
13   GLNPHEASPASPGLUSERALATHRVALVAL
14   ASPPHELEUARGARGTRPILEALAPHECYS
15   GLNSERILEILESERTHRSERPROGLNALA
16   ALAALAHISHISHISHISHISHISHISHIS

Samples:

sample_1: IL-2, [U-13C; U-15N; U-2H; MILV-methyl], 0.6 mM; sodium chloride 150 mM

sample_2: IL-2, [13C MILV methyl; U-15N], 0.4 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSY-HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HMQC methylsample_2isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphatic (CCH)sample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D HMCM(CG)CBCAsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphatic (HCH)sample_2isotropicsample_conditions_1

Software:

CCPN, CCPN, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks