BMRB Entry 27958

Title:
Spin-labeled DNA duplex
Deposition date:
2019-06-24
Original release date:
2020-02-26
Authors:
Lane, Andrew
Citation:

Citation: Hardwick, Jack; Haugland, Marius; El-Sagheer, Afaf; Ptchelkine, Denis; Beierlein, Frank; Lane, Andrew; Brown, Tom; Lovett, Janet; Anderson, Edward. "2'-Alkynyl spin-labelling is a minimally perturbing tool for DNA structural analysis"  Nucleic Acids Res. 48, 2830-2840 (2020).
PubMed: 32052020

Assembly members:

Assembly members:
DNA_strand_A, polymer, 14 residues, Formula weight is not available
DNA_strand_B, polymer, 14 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DNA_strand_A: CTXACGCGTCATTG
DNA_strand_B: CAATGACGCGTAAG

Data typeCount
1H chemical shifts618

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA strand A1
2DNA strand B2

Entities:

Entity 1, DNA strand A 14 residues - Formula weight is not available

1   DCDTDXDADCDGDCDGDTDC
2   DADTDTDG

Entity 2, DNA strand B 14 residues - Formula weight is not available

1   DCDADADTDGDADCDGDCDG
2   DTDADADG

Samples:

sample_1: DNA strand A 0.75 mM; DNA strand B 0.75 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 30 K

Experiments:

NameSampleSample stateSample conditions
Presatsample_1isotropicsample_conditions_1
2D 2Q COSYsample_1isotropicsample_conditions_1
2D NOESYsample_1isotropicsample_conditions_1
2D NOESYsample_1isotropicsample_conditions_1
1D water suppressionsample_1isotropicsample_conditions_1

Software:

VNMRJ, Agilent - data analysis

NMR spectrometers:

  • Agilent DD2 600 MHz