BMRB Entry 27868

Title:
Backbone 1H, 13C and 15N chemical shift assignment for CHIKV HVD
Deposition date:
2019-04-04
Original release date:
2020-02-26
Authors:
Agback, Peter
Citation:

Citation: Agback, Peter; Dominguez, Francisco; Pustovalova, Yulia; Lukash, Tetyana; Shiliaev, Nikita; Orekhov, Vladislav; Frolov, Ilya; Agback, Tatiana; Frolova, Elena. "Structural characterization and biological function of bivalent binding of CD2AP to intrinsically disordered domain of chikungunya virus nsP3 protein"  Virology 537, 130-142 (2019).
PubMed: 31493651

Assembly members:

Assembly members:
CHIKV_HVD, polymer, 230 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pE-SUMOpro-3

Data sets:
Data typeCount
13C chemical shifts633
15N chemical shifts201
1H chemical shifts375

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CHIKV protein1

Entities:

Entity 1, CHIKV protein 230 residues - Formula weight is not available

1   GLYARGSERSERGLNGLUSERVALGLNGLU
2   VALSERTHRTHRTHRSERLEUTHRHISSER
3   GLNPHEASPLEUSERALAASPGLYGLUTHR
4   LEUPROVALPROSERASPLEUASPALAASP
5   ALAPROALALEUGLUPROALALEUASPASP
6   GLYALAVALHISTHRLEUPROTHRILEILE
7   GLYASNLEUALAALAVALSERASPTRPVAL
8   METSERTHRVALPROVALALAPROPROARG
9   ARGARGARGGLYARGASNLEUTHRVALTHR
10   CYSASPGLUARGGLUGLYASNILETHRPRO
11   METALASERVALARGPHEPHEARGALAGLU
12   LEUCYSPROALAVALGLNGLUTHRALAGLU
13   THRARGASPTHRALAILESERLEUGLNALA
14   PROPROSERTHRTHRMETGLULEUSERHIS
15   PROPROILESERPHEGLYALAPROSERGLU
16   THRPHEPROILETHRPHEGLYASPPHEASP
17   GLUGLYGLUILEGLUSERLEUSERSERGLU
18   LEULEUTHRPHEGLYASPPHELEUPROGLY
19   GLUVALASPASPLEUTHRASPSERASPTRP
20   SERTHRCYSPROASPTHRASPASPGLULEU
21   GLYSERTRPSERHISPROGLNPHEGLULYS
22   GLYGLYGLYSERGLYGLYGLYSERGLYGLY
23   GLYSERTRPSERHISPROGLNPHEGLULYS

Samples:

sample_1: CHIKV HVD, [U-99% 13C; U-99% 15N], 0.27 mM; sodium chloride 50 mM; TCEP 2 mM; sodium phosphate 25 mM; sodium azide 1 mM; D2O, [U-99% 2H], 10%

sample_conditions_1: ionic strength: 200 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.5, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks