BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27856

Title: Resonance assignments of N terminal Receiver domain of Sigma factor S regulator RssB from Escherichia coli   PubMed: 31228091

Deposition date: 2019-03-29 Original release date: 2019-06-26

Authors: Liu, Bing; Wang, Zhihao

Citation: Wang, Zhihao; Zhao, Siyu; Jiang, Songzi; Wang, Yawen; Buck, Martin; Matthews, Steve; Liu, Bing. "Resonance assignments of N-terminal receiver domain of sigma factor S regulator RssB from Escherichia coli."  Biomol. NMR Assign. 13, 333-337 (2019).

Assembly members:
RssB_N-terminal_1-125, polymer, 125 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
RssB_N-terminal_1-125: MTQPLVGKQILIVEDEQVFR SLLDSWFSSLGATTVLAADG VDALELLGGFTPDLMICDIA MPRMNGLKLLEHIRNRGDQT PVLVISATENMADIAKALRL GVEDVLLKPVKDLNRLREMV FACLY

Data sets:
Data typeCount
13C chemical shifts507
15N chemical shifts126
1H chemical shifts897

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RssB1

Entities:

Entity 1, RssB 125 residues - Formula weight is not available

1   METTHRGLNPROLEUVALGLYLYSGLNILE
2   LEUILEVALGLUASPGLUGLNVALPHEARG
3   SERLEULEUASPSERTRPPHESERSERLEU
4   GLYALATHRTHRVALLEUALAALAASPGLY
5   VALASPALALEUGLULEULEUGLYGLYPHE
6   THRPROASPLEUMETILECYSASPILEALA
7   METPROARGMETASNGLYLEULYSLEULEU
8   GLUHISILEARGASNARGGLYASPGLNTHR
9   PROVALLEUVALILESERALATHRGLUASN
10   METALAASPILEALALYSALALEUARGLEU
11   GLYVALGLUASPVALLEULEULYSPROVAL
12   LYSASPLEUASNARGLEUARGGLUMETVAL
13   PHEALACYSLEUTYR

Samples:

sample_1: RssB N-terminal 1-125, [U-100% 13C; U-100% 15N], 0.5 mM; sodium chloride 250 mM; TRIS 50 mM; TCEP 1 mM

sample_2: RssB N-terminal 1-125, [U-100% 13C; U-100% 15N; U-80% 2H], 0.5 mM; sodium chloride 250 mM; TRIS 50 mM; TCEP 1 mM

sample_conditions_1: ionic strength: 250 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 900 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts