BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27848

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments of co-repressor nuclear receptor interacting domain N-CoR NID   PubMed: 31178221

Deposition date: 2019-03-21 Original release date: 2019-07-25

Authors: Barthe, Philippe; Sibille, Nathalie; Bernado, Pau

Citation: Cordeiro, Tiago; Sibille, Nathalie; Germain, Pierre; Barthe, Philippe; Boulahtouf, Abdelhay; Allemand, Frederic; Bailly, Remy; Vivat, Valerie; Ebel, Christine; Barducci, Alessandro; Bourguet, William; le Maire, Albane; Bernado, Pau. "Interplay of Protein Disorder in Retinoic Acid Receptor Heterodimer and Its Corepressor Regulates Gene Expression"  Structure 27, 1270-1285 (2019).

Assembly members:
N-CoR_NID, polymer, 269 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
N-CoR_NID: GSQVPRTHRLITLADHICQI ITQDFARNQVPSQPSTSTFQ TSPSALSSTPVRTKTSSRYS PESQSQTVLHPRPGPRVSPE NLVDKSRGSRPGKSPERSHI PSEPYEPISPPQGPAVHEKQ DSMLLLSQRGVDPAEQRSDS RSPGSISYLPSFFTKLESTS PMVKSKKQEIFRKLNSSGGG DSDMAAAQPGTEIFNLPAVT TSGAVSSRSHSFADPASNLG LEDIIRKALMGSFDDKVEDH GVVMSHPVGIMPGSASTSVV TSSEARRDE

Data sets:
Data typeCount
13C chemical shifts617
15N chemical shifts183
1H chemical shifts183

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1N-CoR NID1

Entities:

Entity 1, N-CoR NID 269 residues - Formula weight is not available

Nuclear receptor corepressor 1 (N-CoR) Nuclear Receptor Interacting Domain (NID).

1   GLYSERGLNVALPROARGTHRHISARGLEU
2   ILETHRLEUALAASPHISILECYSGLNILE
3   ILETHRGLNASPPHEALAARGASNGLNVAL
4   PROSERGLNPROSERTHRSERTHRPHEGLN
5   THRSERPROSERALALEUSERSERTHRPRO
6   VALARGTHRLYSTHRSERSERARGTYRSER
7   PROGLUSERGLNSERGLNTHRVALLEUHIS
8   PROARGPROGLYPROARGVALSERPROGLU
9   ASNLEUVALASPLYSSERARGGLYSERARG
10   PROGLYLYSSERPROGLUARGSERHISILE
11   PROSERGLUPROTYRGLUPROILESERPRO
12   PROGLNGLYPROALAVALHISGLULYSGLN
13   ASPSERMETLEULEULEUSERGLNARGGLY
14   VALASPPROALAGLUGLNARGSERASPSER
15   ARGSERPROGLYSERILESERTYRLEUPRO
16   SERPHEPHETHRLYSLEUGLUSERTHRSER
17   PROMETVALLYSSERLYSLYSGLNGLUILE
18   PHEARGLYSLEUASNSERSERGLYGLYGLY
19   ASPSERASPMETALAALAALAGLNPROGLY
20   THRGLUILEPHEASNLEUPROALAVALTHR
21   THRSERGLYALAVALSERSERARGSERHIS
22   SERPHEALAASPPROALASERASNLEUGLY
23   LEUGLUASPILEILEARGLYSALALEUMET
24   GLYSERPHEASPASPLYSVALGLUASPHIS
25   GLYVALVALMETSERHISPROVALGLYILE
26   METPROGLYSERALASERTHRSERVALVAL
27   THRSERSERGLUALAARGARGASPGLU

Samples:

sample_1: N-CoR NID, [U-100% 13C; U-100% 15N], 600 uM; TRIS Bis 50 mM; NaCl 150 mM; EDTA 2 mM; TCEP 2 mM

N-CoR_NID_buffer: ionic strength: 150 mM; pH: 6.7; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicN-CoR_NID_buffer
3D BT-HNCOsample_1isotropicN-CoR_NID_buffer
3D BT-HN(CA)COsample_1isotropicN-CoR_NID_buffer
3D BT-HNCAsample_1isotropicN-CoR_NID_buffer
3D BT-HN(CO)CAsample_1isotropicN-CoR_NID_buffer
3D BT-HNCACBsample_1isotropicN-CoR_NID_buffer
3D BT-HN(CO)CACBsample_1isotropicN-CoR_NID_buffer
3D BT-iHNCAsample_1isotropicN-CoR_NID_buffer
3D BT-HN(COCA)NHsample_1isotropicN-CoR_NID_buffer
3D BT-HN(CA)NHsample_1isotropicN-CoR_NID_buffer

Software:

TOPSPIN, Bruker Biospin - collection

GIFA, Delsuc - chemical shift calculation, processing

CINDY, Andre PADILLA (CBS) - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 950 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts