BMRB Entry 27827

Title:
Human IMP3 KH1-2 delta 1 amide chemical shifts
Deposition date:
2019-03-06
Original release date:
2019-03-07
Authors:
Schlundt, Andreas; Sattler, Michael; Aziz, Masood; Wagner, Jacqueline
Citation:

Citation: Schneider, Tim; Hung, Lee-Hsueh; Aziz, Masood; Wilmen, Anna; Thaum, Stephanie; Wagner, Jacqueline; Janowski, Robert; Mueller, Simon; Schreiner, Silke; Friedhoff, Peter; Huettelmaier, Stefan; Niessing, Dierk; Sattler, Michael; Schlundt, Andreas; Bindereif, Albrecht. "Combinatorial recognition of clustered RNA elements by the multidomain RNA-binding protein IMP3."  Nat. Commun. 10, 2266-2266 (2019).
PubMed: 31118463

Assembly members:

Assembly members:
IMP3_(IGF2BP3)_KH1-2_delta1, polymer, 168 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETTrx1a

Data sets:
Data typeCount
15N chemical shifts145
1H chemical shifts145

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IMP3 KH1-2 delta11

Entities:

Entity 1, IMP3 KH1-2 delta1 168 residues - Formula weight is not available

GAMA is artefact from cloning

1   GLYALAMETALALYSPROCYSASPLEUPRO
2   LEUARGLEULEUVALPROTHRGLNPHEVAL
3   GLYALAILEILEGLYASPASPGLYALATHR
4   ILEARGASNILETHRLYSGLNTHRGLNSER
5   LYSILEASPVALHISARGLYSGLUASNALA
6   GLYALAALAGLULYSSERILETHRILELEU
7   SERTHRPROGLUGLYTHRSERALAALACYS
8   LYSSERILELEUGLUILEMETHISLYSGLU
9   ALAGLNASPILELYSPHETHRGLUGLUILE
10   PROLEULYSILELEUALAHISASNASNPHE
11   VALGLYARGLEUILEGLYLYSGLUGLYARG
12   ASNLEULYSLYSILEGLUGLNASPTHRASP
13   THRLYSILETHRILESERPROLEUGLNGLU
14   LEUTHRLEUTYRASNPROGLUARGTHRILE
15   THRVALLYSGLYASNVALGLUTHRCYSALA
16   LYSALAGLUGLUGLUILEMETLYSLYSILE
17   ARGGLUSERTYRGLUASNASPILE

Samples:

sample_1: IMP3 (IGF2BP3) KH1-2 delta1, [U-15N], 0.125 mM; BisTris 20 mM; sodium chloride 150 mM; TCEP 2 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker - collection, processing

CCPNMR_Analysis, CCPN - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz
  • Bruker Avance 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks