BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27795

Title: FLNa4-6   PubMed: 31542223

Deposition date: 2019-02-21 Original release date: 2020-02-28

Authors: Haataja, Tatu; Permi, Perttu

Citation: Haataja, Tatu; Capoulade, Romain; Lecointe, Simon; Hellman, Maarit; Merot, Jean; Permi, Perttu; Pentikainen, Ulla. "Critical Structural Defects Explain Filamin A Mutations Causing Mitral Valve Dysplasia"  Biophys. J. 117, 1467-1475 (2019).

Assembly members:
FLNa4-6, polymer, 298 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
FLNa4-6: SMCGNQKVRAWGPGLEGGVV GKSADFVVEAIGDDVGTLGF SVEGPSQAKIECDDKGDGSC DVRYWPQEAGEYAVHVLCNS EDIRLSPFMADIRDAPQDFH PDRVKARGPGLEKTGVAVNK PAEFTVDAKHGGKAPLRVQV QDNEGCPVEALVKDNGNGTY SCSYVPRKPVKHTAMVSWGG VSIPNSPFRVNVGAGSHPNK VKVYGPGVAKTGLKAHEPTY FTVDCAEAGQGDVSIGIKCA PGVVGPAEADIDFDIIRNDN DTFTVKYTPRGAGSYTIMVL FADQATPTSPIRVKVEPS

Data sets:
Data typeCount
13C chemical shifts556
15N chemical shifts245
1H chemical shifts305

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FLNa4-61

Entities:

Entity 1, FLNa4-6 298 residues - Formula weight is not available

1   SERMETCYSGLYASNGLNLYSVALARGALA
2   TRPGLYPROGLYLEUGLUGLYGLYVALVAL
3   GLYLYSSERALAASPPHEVALVALGLUALA
4   ILEGLYASPASPVALGLYTHRLEUGLYPHE
5   SERVALGLUGLYPROSERGLNALALYSILE
6   GLUCYSASPASPLYSGLYASPGLYSERCYS
7   ASPVALARGTYRTRPPROGLNGLUALAGLY
8   GLUTYRALAVALHISVALLEUCYSASNSER
9   GLUASPILEARGLEUSERPROPHEMETALA
10   ASPILEARGASPALAPROGLNASPPHEHIS
11   PROASPARGVALLYSALAARGGLYPROGLY
12   LEUGLULYSTHRGLYVALALAVALASNLYS
13   PROALAGLUPHETHRVALASPALALYSHIS
14   GLYGLYLYSALAPROLEUARGVALGLNVAL
15   GLNASPASNGLUGLYCYSPROVALGLUALA
16   LEUVALLYSASPASNGLYASNGLYTHRTYR
17   SERCYSSERTYRVALPROARGLYSPROVAL
18   LYSHISTHRALAMETVALSERTRPGLYGLY
19   VALSERILEPROASNSERPROPHEARGVAL
20   ASNVALGLYALAGLYSERHISPROASNLYS
21   VALLYSVALTYRGLYPROGLYVALALALYS
22   THRGLYLEULYSALAHISGLUPROTHRTYR
23   PHETHRVALASPCYSALAGLUALAGLYGLN
24   GLYASPVALSERILEGLYILELYSCYSALA
25   PROGLYVALVALGLYPROALAGLUALAASP
26   ILEASPPHEASPILEILEARGASNASPASN
27   ASPTHRPHETHRVALLYSTYRTHRPROARG
28   GLYALAGLYSERTYRTHRILEMETVALLEU
29   PHEALAASPGLNALATHRPROTHRSERPRO
30   ILEARGVALLYSVALGLUPROSER

Samples:

sample_1: FLNa4-6, [U-100% 13C; U-100% 15N], 0.8 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

UNP P21333

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts