BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27782

Title: 1HN, 13C, and 15N Resonance Assignments of human Calmodulin bound to CaMBP2 peptide   PubMed: 30875027

Deposition date: 2019-02-12 Original release date: 2019-04-12

Authors: Varney, Kristen; Wilder, Paul; Raquel, Godoy-Ruiz; Mancia, Filippo; Weber, David

Citation: Varney, Kristen; Wilder, Paul; Godoy-Ruiz, Raquel; Mancia, Filippo; Weber, David. "1HN, 13C, and 15N resonance assignments of human calmodulin bound to a peptide derived from the STRA6 vitamin A transporter (CaMBP2)"  Biomol. NMR Assignments 13, 275-278 (2019).

Assembly members:
Calmodulin, polymer, 148 residues, Formula weight is not available
CaMBP2, polymer, 27 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Calmodulin: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
CaMBP2: VSNAKRARAHWQLLYTLVNN PSLVGSR

Data sets:
Data typeCount
13C chemical shifts219
15N chemical shifts131
1H chemical shifts130

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Calmodulin1
2CaMBP22

Entities:

Entity 1, Calmodulin 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, CaMBP2 27 residues - Formula weight is not available

1   VALSERASNALALYSARGALAARGALAHIS
2   TRPGLNLEULEUTYRTHRLEUVALASNASN
3   PROSERLEUVALGLYSERARG

Samples:

sample_1: Calmodulin, [U-13C; U-15N], 0.25 mM; CaMBP2 0.25 mM; sodium chloride 50 mM; calcium chloride 10 mM; magnesium chloride 5 mM; TCEP 0.5 mM

sample_conditions_1: ionic strength: 80 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance III 950 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts