BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27775

Title: HLS3_Olduvai   PubMed: 31264103

Authors: Henen, Morkos; Schmitt, Lauren; Issaian, Aaron; Vogeli, Beat; Hansen, Kirk

Citation: Issaian, Aaron; Schmitt, Lauren; Born, Alexandra; Nichols, Parker; Sikela, James; Hansen, Kirk; Vogeli, Beat; Henen, Morkos. "Solution NMR backbone assignment reveals interaction-free tumbling of human lineage-specific Olduvai protein domains"  Biomol. NMR Assign. 13, 339-343 (2019).

Assembly members:
HLS3, polymer, 94 residues, 10553.3948 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
HLS3: SAAAASSASLEVDRIKKDQE EEEDQGPPCPRLSRELLEVV EPEVLQDSLDRCYSTPSSCL EQPDSCQPYGSSFYALEEKH VGFSLDLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts188
15N chemical shifts64
1H chemical shifts64

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HLS31

Entities:

Entity 1, HLS3 94 residues - 10553.3948 Da.

1   SERALAALAALAALASERSERALASERLEU
2   GLUVALASPARGILELYSLYSASPGLNGLU
3   GLUGLUGLUASPGLNGLYPROPROCYSPRO
4   ARGLEUSERARGGLULEULEUGLUVALVAL
5   GLUPROGLUVALLEUGLNASPSERLEUASP
6   ARGCYSTYRSERTHRPROSERSERCYSLEU
7   GLUGLNPROASPSERCYSGLNPROTYRGLY
8   SERSERPHETYRALALEUGLUGLULYSHIS
9   VALGLYPHESERLEUASPLEUGLUHISHIS
10   HISHISHISHIS

Samples:

sample_1: HLS3, [U-100% 13C; U-100% 15N], 140 uM; sodium chloride 50 mM; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
Expt_3 (H[N[co[{CA|ca[C]}]]])sample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

CcpNmr_Analysis v2.4, CCPN, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - chemical shift assignment, data analysis, processing

NMR spectrometers:

  • Varian INOVA 900 MHz
  • Varian INOVA 600 MHz

Related Database Links: