BMRB Entry 27761

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for ARNO Sec7
Published: 2020-02-28

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27761

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for ARNO Sec7

Deposition date:

2019-01-22

Original release date:

2020-02-28

Authors:

Etzkorn, Manuel; Viegas, Aldino; Yin, Dongsheng; Borggraefe, Jan; Viennet, Thibault; Falke, Marcel; Schmitz, Anton; Famulok, Michael

Citation:

Citation: Viegas, Aldino; Yin, Dongsheng; Borggraefe, Jan; Viennet, Thibault; Falke, Marcel; Schmitz, Anton; Famulok, Michael; Etzkorn, Manuel. "Molecular Architecture of a Network of Potential Intracellular EGFR Modulators: ARNO, CaM, Phospholipids, and the Juxtamembrane Segment" Structure 28, 54-62.e5 (2020).
PubMed: 31780432

Assembly members:

Assembly members:
Sec7, polymer, 189 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sec7: SETRNRKMAMGRKKFNMDPK KGIQFLVENELLQNTPEEIA RFLYKGEGLNKTAIGDYLGE REELNLAVLHAFVDLHEFTD LNLVQALRQFLWSFRLPGEA QKIDRMMEAFAQRYCLCNPG VFQSTDTCYVLSFAVIMLNT SLHNPNVRDKPGLERFVAMN RGINEGGDLPEELLRNLYDS IRNEPFKIP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	483
15N chemical shifts	150
1H chemical shifts	150

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ARNO-Sec7	1

Entities:

Entity 1, ARNO-Sec7 189 residues - Formula weight is not available

Residues 58-60 represent a non-native tag

1

SER

GLU

THR

ARG

ASN

ARG

LYS

MET

ALA

MET

2

GLY

ARG

LYS

PHE

ASN

MET

ASP

PRO

LYS

3

LYS

GLY

ILE

GLN

PHE

LEU

VAL

GLU

ASN

GLU

4

LEU

GLN

ASN

THR

PRO

GLU

ILE

ALA

5

ARG

PHE

LEU

TYR

LYS

GLY

GLU

GLY

LEU

ASN

6

LYS

THR

ALA

ILE

GLY

ASP

TYR

LEU

GLY

GLU

7

ARG

GLU

LEU

ASN

LEU

ALA

VAL

LEU

HIS

8

ALA

PHE

VAL

ASP

LEU

HIS

GLU

PHE

THR

ASP

9

LEU

ASN

LEU

VAL

GLN

ALA

LEU

ARG

GLN

PHE

10

LEU

TRP

SER

PHE

ARG

LEU

PRO

GLY

GLU

ALA

11

GLN

LYS

ILE

ASP

ARG

MET

GLU

ALA

PHE

12

ALA

GLN

ARG

TYR

CYS

LEU

CYS

ASN

PRO

GLY

13

VAL

PHE

GLN

SER

THR

ASP

THR

CYS

TYR

VAL

14

LEU

SER

PHE

ALA

VAL

ILE

MET

LEU

ASN

THR

15

SER

LEU

HIS

ASN

PRO

ASN

VAL

ARG

ASP

LYS

16

PRO

GLY

LEU

GLU

ARG

PHE

VAL

ALA

MET

ASN

17

ARG

GLY

ILE

ASN

GLU

GLY

ASP

LEU

PRO

18

GLU

LEU

ARG

ASN

LEU

TYR

ASP

SER

19

ILE

ARG

ASN

GLU

PRO

PHE

LYS

ILE

PRO

Samples:

sample_1: Sec7, [U-13C; U-15N; U-2H], 0.36 mM; sodium phosphate 20 mM; DSS 0.05 mM; sodium azide 0.01%; sodium chloride 300 mM; H2O 90 % v/v; D2O 10 % v/v

sample_conditions_1: ionic strength: 300 mM; pH: 7.4; pressure: 1 atm; temperature: 305.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.9.24a, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks