BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27664

Title: UbcH7-Ub isopeptide conjugate

Authors: Condos, Tara; Shaw, Gary

Citation: Condos, Tara; Shaw, Gary. "Synergistic Recruitment of UbcH7~Ub and Phosphorylated Ubl Domain Triggers Parkin Activation"  EMBO J. ., .-..

Assembly members:
UbcH7, polymer, 154 residues, Formula weight is not available
Ub, polymer, 76 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
UbcH7: MAASRRLMKELEEIRKSGMK NFRNIQVDEANLLTWQGLIV PDNPPYDKGAFRIEINFPAE YPFKPPKITFKTKIYHPNID EKGQVKLPVISAENWKPATK TDQVIQSLIALVNDPQPEHP LRADLAEEYSKDRKKFSKNA EEFTKKYGEKRPVD
Ub: MQIFVKTLTGKTITLEVESS DTIDNVKSKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGG

Data sets:
Data typeCount
15N chemical shifts208
1H chemical shifts196

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UbcH71
2Ub2

Entities:

Entity 1, UbcH7 154 residues - Formula weight is not available

1   METALAALASERARGARGLEUMETLYSGLU
2   LEUGLUGLUILEARGLYSSERGLYMETLYS
3   ASNPHEARGASNILEGLNVALASPGLUALA
4   ASNLEULEUTHRTRPGLNGLYLEUILEVAL
5   PROASPASNPROPROTYRASPLYSGLYALA
6   PHEARGILEGLUILEASNPHEPROALAGLU
7   TYRPROPHELYSPROPROLYSILETHRPHE
8   LYSTHRLYSILETYRHISPROASNILEASP
9   GLULYSGLYGLNVALLYSLEUPROVALILE
10   SERALAGLUASNTRPLYSPROALATHRLYS
11   THRASPGLNVALILEGLNSERLEUILEALA
12   LEUVALASNASPPROGLNPROGLUHISPRO
13   LEUARGALAASPLEUALAGLUGLUTYRSER
14   LYSASPARGLYSLYSPHESERLYSASNALA
15   GLUGLUPHETHRLYSLYSTYRGLYGLULYS
16   ARGPROVALASP

Entity 2, Ub 76 residues - Formula weight is not available

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUSERSER
3   ASPTHRILEASPASNVALLYSSERLYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLY

Samples:

sample_1: UbcH7, [U-99% 2H], 0.11 ± 0.1 mM; Ub, [U-99% 2H], 0.11 ± 0.1 mM; DSS 0.01 mM; HEPES 25 ± 0.1 mM; sodium chloride 50 mM; TCEP 0.5 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe v9.2, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRViewJ v9.2.0, Johnson, One Moon Scientific - chemical shift assignment, peak picking

VNMRJ v4.2, Varian - collection

NMRView v9.2.0, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15498 4769 15498 4769