BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27641

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for MPS1   PubMed: 30784592

Deposition date: 2018-10-09 Original release date: 2019-02-12

Authors: Hiruma, Yoshitaka; Perrakis, Anastassis

Citation: Pachis, Spyridon; Hiruma, Yoshitaka; Tromer, Eelco; Perrakis, Anastassis; Kops, Geert. "Interactions between N-terminal Modules in MPS1 Enable Spindle Checkpoint Silencing."  Cell Rep. 26, 2101-2112 (2019).

Assembly members:
TTK, polymer, 239 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
TTK: MESEDLSGRELTIDSIMNKV RDIKNKFKNEDLTDELSLNK ISADTTDNSGTVNQIMMMAN NPEDWLSLLLKLEKNSVPLS DALLNKLIGRYSQAIEALPP DKYGQNESFARIQVRFAELK AIQEPDDARDYFQMARANCK KFAFVHISFAQFELSQGNVK KSKQLLQKAVERGAVPLEML EIALRNLNLQKKQLLSEEEK KNLSASTVLTAQESFSGSLG HLQNRNNSCDSRGQTTKAR

Data sets:
Data typeCount
13C chemical shifts617
15N chemical shifts210
1H chemical shifts210

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Mps1 1-2391

Entities:

Entity 1, Mps1 1-239 239 residues - Formula weight is not available

1   METGLUSERGLUASPLEUSERGLYARGGLU
2   LEUTHRILEASPSERILEMETASNLYSVAL
3   ARGASPILELYSASNLYSPHELYSASNGLU
4   ASPLEUTHRASPGLULEUSERLEUASNLYS
5   ILESERALAASPTHRTHRASPASNSERGLY
6   THRVALASNGLNILEMETMETMETALAASN
7   ASNPROGLUASPTRPLEUSERLEULEULEU
8   LYSLEUGLULYSASNSERVALPROLEUSER
9   ASPALALEULEUASNLYSLEUILEGLYARG
10   TYRSERGLNALAILEGLUALALEUPROPRO
11   ASPLYSTYRGLYGLNASNGLUSERPHEALA
12   ARGILEGLNVALARGPHEALAGLULEULYS
13   ALAILEGLNGLUPROASPASPALAARGASP
14   TYRPHEGLNMETALAARGALAASNCYSLYS
15   LYSPHEALAPHEVALHISILESERPHEALA
16   GLNPHEGLULEUSERGLNGLYASNVALLYS
17   LYSSERLYSGLNLEULEUGLNLYSALAVAL
18   GLUARGGLYALAVALPROLEUGLUMETLEU
19   GLUILEALALEUARGASNLEUASNLEUGLN
20   LYSLYSGLNLEULEUSERGLUGLUGLULYS
21   LYSASNLEUSERALASERTHRVALLEUTHR
22   ALAGLNGLUSERPHESERGLYSERLEUGLY
23   HISLEUGLNASNARGASNASNSERCYSASP
24   SERARGGLYGLNTHRTHRLYSALAARG

Samples:

sample_1: TTK, [U-13C; U-15N], 500 uM; HEPES/NaOH 20 mM; KCl 150 mM; TCEP 1 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CCPN, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 850 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts