BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27608

Title: MapZ is a multi-functional regulator of FtsZ that controls both the precise positioning of Z-ring and proper timing of Z-ring formation   PubMed: 31036719

Deposition date: 2018-09-13 Original release date: 2019-05-24

Authors: Feng, Zhang; Zhang, Jiahai; Xu, Da; Jiang, Yongliang; Zhou, Congzhao; Chen, Yuxing

Citation: Feng, Zhang; Zhang, Jiahai; Xu, Da; Jiang, Yongliang; Zhou, Congzhao; Chen, Yuxing. "Multi-functional regulator MapZ controls both positioning and timing of FtsZ polymerization."  Biochem. J. 476, 1433-1444 (2019).

Assembly members:
MapZ, polymer, 158 residues, Formula weight is not available

Natural source:   Common Name: S. pneumoniae   Taxonomy ID: 1313   Superkingdom: Bacteria   Kingdom: Bacteria   Genus/species: Streptococcus pneumoniae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
MapZ: MSKKRRNRHKKEAQEPQFDF DEAKELTVGQAIRKNEEVEA GVLPEDSILDKYVKQHRDEI EADKFATRQYKKEEFVETQS LDDLIQEMREAVEKSEASSE EVPSSEDILLPLPLDDEEQG LDPLLLDDENPTEMTEEVEE EQNLSRLDQEDSEKKSKK

Data sets:
Data typeCount
13C chemical shifts641
15N chemical shifts163
1H chemical shifts1015

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MapZ1

Entities:

Entity 1, MapZ 158 residues - Formula weight is not available

1   METSERLYSLYSARGARGASNARGHISLYS
2   LYSGLUALAGLNGLUPROGLNPHEASPPHE
3   ASPGLUALALYSGLULEUTHRVALGLYGLN
4   ALAILEARGLYSASNGLUGLUVALGLUALA
5   GLYVALLEUPROGLUASPSERILELEUASP
6   LYSTYRVALLYSGLNHISARGASPGLUILE
7   GLUALAASPLYSPHEALATHRARGGLNTYR
8   LYSLYSGLUGLUPHEVALGLUTHRGLNSER
9   LEUASPASPLEUILEGLNGLUMETARGGLU
10   ALAVALGLULYSSERGLUALASERSERGLU
11   GLUVALPROSERSERGLUASPILELEULEU
12   PROLEUPROLEUASPASPGLUGLUGLNGLY
13   LEUASPPROLEULEULEUASPASPGLUASN
14   PROTHRGLUMETTHRGLUGLUVALGLUGLU
15   GLUGLNASNLEUSERARGLEUASPGLNGLU
16   ASPSERGLULYSLYSSERLYSLYS

Samples:

sample_1: MapZ, [U-99% 13C; U-99% 15N], 0.8 mM; phosphate sodium 50 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts