BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27588

Title: Assignment of E.coli asparaginase (ANSII) by solution NMR   PubMed: 30462348

Deposition date: 2018-08-24 Original release date: 2018-11-28

Authors: Cerofolini, Linda; Giuntini, Stefano; Carlon, Azzurra; Ravera, Enrico; Calderone, Vito; Fragai, Marco; Parigi, Giacomo; Luchinat, Claudio

Citation: Cerofolini, Linda; Giuntini, Stefano; Carlon, Azzurra; Ravera, Enrico; Calderone, Vito; Fragai, Marco; Parigi, Giacomo; Luchinat, Claudio. "Characterization of PEGylated asparaginase: new opportunities from NMR analysis of large pegylated therapeutics"  Chemistry 25, 1984-1991 (2019).

Assembly members:
ANSII, polymer, 326 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ANSII: LPNITILATGGTIAGGGDSA TKSNYTVGKVGVENLVNAVP QLKDIANVKGEQVVNIGSQD MNDNVWLTLAKKINTDCDKT DGFVITHGTDTMEETAYFLD LTVKCDKPVVMVGAMRPSTS MSADGPFNLYNAVVTAADKA SANRGVLVVMNDTVLDGRDV TKTNTTDVATFKSVNYGPLG YIHNGKIDYQRTPARKHTSD TPFDVSKLNELPKVGIVYNY ANASDLPAKALVDAGYDGIV SAGVGNGNLYKSVFDTLATA AKTGTAVVRSSRVPTGATTQ DAEVDDAKYGFVASGTLNPQ KARVLLQLALTQTKDPQQIQ QIFNQY

Data sets:
Data typeCount
13C chemical shifts561
15N chemical shifts186
1H chemical shifts340

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ANSII, chain A1
2ANSII, chain B1
3ANSII, chain C1
4ANSII, chain D1

Entities:

Entity 1, ANSII, chain A 326 residues - Formula weight is not available

1   LEUPROASNILETHRILELEUALATHRGLY
2   GLYTHRILEALAGLYGLYGLYASPSERALA
3   THRLYSSERASNTYRTHRVALGLYLYSVAL
4   GLYVALGLUASNLEUVALASNALAVALPRO
5   GLNLEULYSASPILEALAASNVALLYSGLY
6   GLUGLNVALVALASNILEGLYSERGLNASP
7   METASNASPASNVALTRPLEUTHRLEUALA
8   LYSLYSILEASNTHRASPCYSASPLYSTHR
9   ASPGLYPHEVALILETHRHISGLYTHRASP
10   THRMETGLUGLUTHRALATYRPHELEUASP
11   LEUTHRVALLYSCYSASPLYSPROVALVAL
12   METVALGLYALAMETARGPROSERTHRSER
13   METSERALAASPGLYPROPHEASNLEUTYR
14   ASNALAVALVALTHRALAALAASPLYSALA
15   SERALAASNARGGLYVALLEUVALVALMET
16   ASNASPTHRVALLEUASPGLYARGASPVAL
17   THRLYSTHRASNTHRTHRASPVALALATHR
18   PHELYSSERVALASNTYRGLYPROLEUGLY
19   TYRILEHISASNGLYLYSILEASPTYRGLN
20   ARGTHRPROALAARGLYSHISTHRSERASP
21   THRPROPHEASPVALSERLYSLEUASNGLU
22   LEUPROLYSVALGLYILEVALTYRASNTYR
23   ALAASNALASERASPLEUPROALALYSALA
24   LEUVALASPALAGLYTYRASPGLYILEVAL
25   SERALAGLYVALGLYASNGLYASNLEUTYR
26   LYSSERVALPHEASPTHRLEUALATHRALA
27   ALALYSTHRGLYTHRALAVALVALARGSER
28   SERARGVALPROTHRGLYALATHRTHRGLN
29   ASPALAGLUVALASPASPALALYSTYRGLY
30   PHEVALALASERGLYTHRLEUASNPROGLN
31   LYSALAARGVALLEULEUGLNLEUALALEU
32   THRGLNTHRLYSASPPROGLNGLNILEGLN
33   GLNILEPHEASNGLNTYR

Samples:

sample_1: ANSII, [U-100% 2H; U-100% 13C; U-100% 15N], 0.75 mM; sodium phosphate 20 mM; sodium azide 0.02%; pefabloc 0.1 mg/mL

sample_2: ANSII, [U-100% 2H; Lys U-100% 1H, 13C, 15N], 0.75 mM; sodium phosphate 20 mM; sodium azide 0.02%; pefabloc 0.1 mg/mL

sample_conditions_1: ionic strength: 0.02 M; pH: 7.5; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSY HSQCsample_1isotropicsample_conditions_1
3D tr-HNCAsample_1isotropicsample_conditions_1
3D tr-HNCACBsample_1isotropicsample_conditions_1
3D tr-HNCOsample_1isotropicsample_conditions_1
3D tr-HN(CA)COsample_1isotropicsample_conditions_1
3D 1H-15N TROSY-NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-1H-1H NOESY-NOESYsample_2isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 950 MHz
  • Bruker Avance 900 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts