BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27578

Title: Putative methyltransferase WBSCR27 in apo-form.   PubMed: 32255258

Deposition date: 2018-08-14 Original release date: 2020-05-25

Authors: Mariasina, Sofia; Polshakov, Vladimir; Chang, Chi-Fon

Citation: Mariasina, Sofia; Chang, Chi-Fon; Petrova, Olga; Efimov, Sergey; Klochkov, Vladimir; Kechko, Olga; Mitkevich, Vladimir; Sergiev, Petr; Dontsova, Olga; Polshakov, Vladimir. "Williams-Beuren Syndrome-Related Methyltransferase WBSCR27: Cofactor Binding and Cleavage"  FEBS J. ., .-..

Assembly members:
WBSCR27, polymer, 240 residues, 25963 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
WBSCR27: GAMAQEEAGRLPQVLARVGT SHGITDLACKLRFYDDWAPE YDQDVAALKYRAPRLAVDCL SRAFRGSPHDALILDVACGT GLVAVELQARGFLQVQGVDG SPEMLKQARARGLYHHLSLC TLGQEPLPDPEGTFDAVIIV GALSEGQVPCSAIPELLRVT KPGGLVCLTTRTNPSNLPYK ETLEATLDSLERAGVWECLV TQPVDHWELATSEQETGLGT CANDGFISGIIYLYRKQETV

Data sets:
Data typeCount
13C chemical shifts612
15N chemical shifts194
1H chemical shifts456

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1WBSCR271

Entities:

Entity 1, WBSCR27 240 residues - 25963 Da.

Residues 1-2 represent a non-native affinity tag

1   GLYALAMETALAGLNGLUGLUALAGLYARG
2   LEUPROGLNVALLEUALAARGVALGLYTHR
3   SERHISGLYILETHRASPLEUALACYSLYS
4   LEUARGPHETYRASPASPTRPALAPROGLU
5   TYRASPGLNASPVALALAALALEULYSTYR
6   ARGALAPROARGLEUALAVALASPCYSLEU
7   SERARGALAPHEARGGLYSERPROHISASP
8   ALALEUILELEUASPVALALACYSGLYTHR
9   GLYLEUVALALAVALGLULEUGLNALAARG
10   GLYPHELEUGLNVALGLNGLYVALASPGLY
11   SERPROGLUMETLEULYSGLNALAARGALA
12   ARGGLYLEUTYRHISHISLEUSERLEUCYS
13   THRLEUGLYGLNGLUPROLEUPROASPPRO
14   GLUGLYTHRPHEASPALAVALILEILEVAL
15   GLYALALEUSERGLUGLYGLNVALPROCYS
16   SERALAILEPROGLULEULEUARGVALTHR
17   LYSPROGLYGLYLEUVALCYSLEUTHRTHR
18   ARGTHRASNPROSERASNLEUPROTYRLYS
19   GLUTHRLEUGLUALATHRLEUASPSERLEU
20   GLUARGALAGLYVALTRPGLUCYSLEUVAL
21   THRGLNPROVALASPHISTRPGLULEUALA
22   THRSERGLUGLNGLUTHRGLYLEUGLYTHR
23   CYSALAASNASPGLYPHEILESERGLYILE
24   ILETYRLEUTYRARGLYSGLNGLUTHRVAL

Samples:

WB_NC: WBSCR27 putative methyltransferase, [U-99% 13C; U-99% 15N], 0.4 mM; NaCl 50 mM; sodium phosphate buffer (pH 7) 50 mM; DTT 10 mM; Sodium azide (NaN3) 0.02%

WB_N: WBSCR27 putative methyltransferase, [U-99% 15N], 0.8 mM; NaCl 50 mM; sodium phosphate buffer (pH 7) 50 mM; DTT 10 mM; Sodium azide (NaN3) 0.02%

sample_conditions_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCWB_Nisotropicsample_conditions_1
2D 1H-13C HSQCWB_NCisotropicsample_conditions_1
3D HNCOWB_NCisotropicsample_conditions_1
3D HCACOWB_NCisotropicsample_conditions_1
3D HN(CO)CAWB_NCisotropicsample_conditions_1
3D HNCAWB_NCisotropicsample_conditions_1
3D CBCA(CO)NHWB_NCisotropicsample_conditions_1
3D HNCACBWB_NCisotropicsample_conditions_1
3D HBHA(CO)NHWB_Nisotropicsample_conditions_1
3D HNHAHBWB_Nisotropicsample_conditions_1
3D HCCH-TOCSYWB_NCisotropicsample_conditions_1

Software:

SPARKY vNMRFAM, (NMRFAM Sparky) W. Lee, Goddard - chemical shift assignment, data analysis, peak picking

TOPSPIN vv3.1, Bruker Biospin - collection

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

TALOS vTALOS+, Cornilescu, Delaglio and Bax - data analysis

NMRest, Polshakov - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 850 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts