BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27569

Title: Human Lineage Specific 1 domain, NBPF15   PubMed: 31264103

Authors: Issaian, Aaron; Hansen, Kirk; Vogeli, Beat; Sikela, James; Henen, Morkos

Citation: Issaian, Aaron; Schmitt, Lauren; Born, Alexandra; Nichols, Parker; Sikela, James; Hansen, Kirk; Vogeli, Beat; Henen, Morkos. "Solution NMR backbone assignment reveals interaction-free tumbling of human lineage-specific Olduvai protein domains"  Biomol. NMR Assign. 13, 339-343 (2019).

Assembly members:
HLS-1, polymer, 94 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
HLS-1: SAAAASSASLEKEDQEATGP RLSRELLDEKEPEVLQDSLD RCYSTPSGCLELTDSCQPYR SAFYVLEQQRVGLAIDMDEI EKYQEVLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts216
15N chemical shifts73
1H chemical shifts73

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HLS11

Entities:

Entity 1, HLS1 94 residues - Formula weight is not available

Residues 1-11, 87-94 are cloning artifact.

1   SERALAALAALAALASERSERALASERLEU
2   GLULYSGLUASPGLNGLUALATHRGLYPRO
3   ARGLEUSERARGGLULEULEUASPGLULYS
4   GLUPROGLUVALLEUGLNASPSERLEUASP
5   ARGCYSTYRSERTHRPROSERGLYCYSLEU
6   GLULEUTHRASPSERCYSGLNPROTYRARG
7   SERALAPHETYRVALLEUGLUGLNGLNARG
8   VALGLYLEUALAILEASPMETASPGLUILE
9   GLULYSTYRGLNGLUVALLEUGLUHISHIS
10   HISHISHISHIS

Samples:

sample_1: HLS-1, [U-100% 13C; U-100% 15N], 320 uM; sodium phosphate 50 mM; sodium chloride 150 mM; DTT 5 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, peak picking, processing

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Varian INOVA 900 MHz
  • Varian INOVA 600 MHz

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