BMRB Entry 27567
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27567
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Citation: Pham, Vincent; Salguero, Carolina; Khan, Shamsun; Meagher, Jennifer; Brown, William; Humbert, Nicolas; de Rocquigny, Hughes; Smith, Janet; D'Souza, Victoria. "Structural analyses of HIV-1 Tat interactions with cellular 7SK and viral TAR RNAs identifies molecular mimicry" Nat. Commun. ., .-..
Assembly members:
Tat_RNA_Binding_Domain, polymer, 17 residues, Formula weight is not available
Natural source: Common Name: HIV Taxonomy ID: 11676 Superkingdom: Viruses Kingdom: not available Genus/species: Lentivirus HIV-1
Experimental source: Production method: obtained from a vendor Host organism: HIV
Entity Sequences (FASTA):
Tat_RNA_Binding_Domain: GISYGRKKRRQRRRAHQ
| Data type | Count |
| . | . |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tat RBD | 1 |
Entities:
Entity 1, Tat RBD 17 residues - Formula weight is not available
| 1 | GLY | ILE | SER | TYR | GLY | ARG | LYS | LYS | ARG | ARG | ||||
| 2 | GLN | ARG | ARG | ARG | ALA | HIS | GLN |
Samples:
2DD2O: HIV TAR RNA 0.5 mM; Tat RNA Binding Domain 0.5 mM; mM NaCl 70 mM; mM phosphates 10 mM
2DH2O: Tat RNA Binding Domain 0.5 mM; HIV TAR RNA 0.5 mM; mM NaCl 70 mM; mM phosphates 10 mM
HMQC: Tat RNA Binding Domain 0.5 mM; HIV TAR RNA, [U-99% 13C; U-99% 15N], 0.5 mM; mM NaCl 70 mM; mM phosphates 10 mM
DeutD2O: Tat RNA Binding Domain 0.5 mM; HIV TAR RNA, [U-97% 2H], 0.5 mM; mM NaCl 70 mM; mM phosphates 10 mM
HSQC: Tat RNA Binding Domain 0.5 mM; HIV TAR RNA, [U-99% 13C; U-99% 15N], 0.5 mM; mM NaCl 70 mM; mM phosphates 10 mM
DeutH2O: Tat RNA Binding Domain 0.5 mM; HIV TAR RNA, [U-97% 2H], 0.5 mM; mM NaCl 70 mM; mM phosphates 10 mM
sample_conditions_1: ionic strength: 80 mM; pH: 5.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | 2DD2O | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | 2DH2O | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | HMQC | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | HSQC | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | DeutD2O | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | DeutH2O | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - data analysis, processing, structure solution
NMR spectrometers:
- Bruker DMX 700 MHz