BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27465

Title: Sensory rhodopsin II has distinct fast internal motion and residual conformational entropy

Deposition date: 2018-04-23 Original release date: 2020-04-07

Authors: O'Brien, Evan; Stetz, Matthew; Lin, Danny; Wand, Joshua

Citation: O'Brien, Evan; Stetz, Matthew; Lin, Danny; Wand, A. Joshua. "Membrane Proteins Have Distinct Fast Internal Motion and Residual Conformational Entropy"  bioRxiv ., .-..

Assembly members:
pSRII, polymer, 245 residues, Formula weight is not available
entity_RET, non-polymer, 284.436 Da.

Natural source:   Common Name: Natronomonas pharaonis   Taxonomy ID: 2257   Superkingdom: Archaea   Kingdom: not available   Genus/species: Natronomonas pharaonis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
pSRII: MVGLTTLFWLGAIGMLVGTL AFAWAGRDAGSGERRYYVTL VGISGIAAVAYVVMALGVGW VPVAERTVFAPRYIDWILTT PLIVYFLGLLAGLDSREFGI VITLNTVVMLAGFAGAMVPG IERYALFGMGAVAFLGLVYY LVGPMTESASQRSSGIKSLY VRLRNLTVILWAIYPFIWLL GPPGVALLTPTVDVALIVYL DLVTXVGFGFIALDAAATLR AEHGESLAGVDTDAPAVADH HHHHH

Data sets:
Data typeCount
order parameters90

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1pSRII1
2cofactor2

Entities:

Entity 1, pSRII 245 residues - Formula weight is not available

1   METVALGLYLEUTHRTHRLEUPHETRPLEU
2   GLYALAILEGLYMETLEUVALGLYTHRLEU
3   ALAPHEALATRPALAGLYARGASPALAGLY
4   SERGLYGLUARGARGTYRTYRVALTHRLEU
5   VALGLYILESERGLYILEALAALAVALALA
6   TYRVALVALMETALALEUGLYVALGLYTRP
7   VALPROVALALAGLUARGTHRVALPHEALA
8   PROARGTYRILEASPTRPILELEUTHRTHR
9   PROLEUILEVALTYRPHELEUGLYLEULEU
10   ALAGLYLEUASPSERARGGLUPHEGLYILE
11   VALILETHRLEUASNTHRVALVALMETLEU
12   ALAGLYPHEALAGLYALAMETVALPROGLY
13   ILEGLUARGTYRALALEUPHEGLYMETGLY
14   ALAVALALAPHELEUGLYLEUVALTYRTYR
15   LEUVALGLYPROMETTHRGLUSERALASER
16   GLNARGSERSERGLYILELYSSERLEUTYR
17   VALARGLEUARGASNLEUTHRVALILELEU
18   TRPALAILETYRPROPHEILETRPLEULEU
19   GLYPROPROGLYVALALALEULEUTHRPRO
20   THRVALASPVALALALEUILEVALTYRLEU
21   ASPLEUVALTHRXVALGLYPHEGLYPHE
22   ILEALALEUASPALAALAALATHRLEUARG
23   ALAGLUHISGLYGLUSERLEUALAGLYVAL
24   ASPTHRASPALAPROALAVALALAASPHIS
25   HISHISHISHISHIS

Entity 2, cofactor - C20 H28 O - 284.436 Da.

1   RET

Samples:

sample_1: pSRII, [U-100% 15N; U-75% 2H; U-100% 13CH3 ILVM], 0.4 mM; retinal 0.4 mM; sodium phosphate 50 mM; sodium chloride 50 mM; sodium azide 3 mM; DHPC, [U-100% 2H aliphatic], 4 % w/v

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 323 K

Experiments:

NameSampleSample stateSample conditions
SQ 1H-13C CRLXsample_1isotropicsample_conditions_1
MQ 1H-13C CRLXsample_1isotropicsample_conditions_1
15N TROSY T1sample_1isotropicsample_conditions_1
15N TROSY T1rhosample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 750 MHz

Related Database Links:

PDB