BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27445

Title: Backbone Assignment of GII.4 Saga Norovirus Protruding Domain   PubMed: 30071731

Deposition date: 2018-04-10 Original release date: 2018-09-18

Authors: Creutznacher, Robert; Mallagaray, Alvaro; Grimm, Lena; Peters, Thomas

Citation: Bucher, Katharina Susanne; Yan, Hao; Creutznacher, Robert; Ruoff, Kerstin; Mallagaray, Alvaro; Grafmuller, Andrea; Dirks, Jan Sebastian; Kilic, Turgay; Weickert, Sabrina; Rubailo, Anna; Drescher, Malte; Schmidt, Stephan; Hansman, Grant; Peters, Thomas; Uetrecht, Charlotte; Hartmann, Laura. "Fucose-Functionalized Precision Glycomacromolecules Targeting Human Norovirus Capsid Protein."  Biomacromolecules 19, 3714-3724 (2018).

Assembly members:
GII.4 Saga Norovirus Protruding Domain, polymer, 310 residues, 34068.2246 Da.

Natural source:   Common Name: Norovirus   Taxonomy ID: 142786   Superkingdom: Viruses   Kingdom: not available   Genus/species: Norovirus not available

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
GII.4 Saga Norovirus Protruding Domain: GPGSKPFTVPILTVEEMTNS RFPIPLEKLFTGPSGAFVVQ PQNGRCTTDGVLLGTTQLSP VNICTFRGDVTHIAGSRNYT MNLASLNWNNYDPTEEIPAP LGTPDFVGKIQGLLTQTTKG DGSTRGHKATVYTGSAPFTP KLGSVQFSTDTENDFETHQN TKFTPVGVIQDGSTTHRNEP QQWVLPSYSGRNVHNVHLAP AVAPTFPGEQLLFFRSTMPG CSGYPNMDLDCLLPQEWVQH FYQEAAPAQSDVALLRFVNP DTGRVLFECKLHKSGYVTVA HTGQHDLVIPPNGYFRFDSW VNQFYTLAPM

Data sets:
Data typeCount
13C chemical shifts767
15N chemical shifts249
1H chemical shifts249

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GII.4 Saga Norovirus Protruding Domain, chain 11
2GII.4 Saga Norovirus Protruding Domain, chain 21

Entities:

Entity 1, GII.4 Saga Norovirus Protruding Domain, chain 1 310 residues - 34068.2246 Da.

1   GLYPROGLYSERLYSPROPHETHRVALPRO
2   ILELEUTHRVALGLUGLUMETTHRASNSER
3   ARGPHEPROILEPROLEUGLULYSLEUPHE
4   THRGLYPROSERGLYALAPHEVALVALGLN
5   PROGLNASNGLYARGCYSTHRTHRASPGLY
6   VALLEULEUGLYTHRTHRGLNLEUSERPRO
7   VALASNILECYSTHRPHEARGGLYASPVAL
8   THRHISILEALAGLYSERARGASNTYRTHR
9   METASNLEUALASERLEUASNTRPASNASN
10   TYRASPPROTHRGLUGLUILEPROALAPRO
11   LEUGLYTHRPROASPPHEVALGLYLYSILE
12   GLNGLYLEULEUTHRGLNTHRTHRLYSGLY
13   ASPGLYSERTHRARGGLYHISLYSALATHR
14   VALTYRTHRGLYSERALAPROPHETHRPRO
15   LYSLEUGLYSERVALGLNPHESERTHRASP
16   THRGLUASNASPPHEGLUTHRHISGLNASN
17   THRLYSPHETHRPROVALGLYVALILEGLN
18   ASPGLYSERTHRTHRHISARGASNGLUPRO
19   GLNGLNTRPVALLEUPROSERTYRSERGLY
20   ARGASNVALHISASNVALHISLEUALAPRO
21   ALAVALALAPROTHRPHEPROGLYGLUGLN
22   LEULEUPHEPHEARGSERTHRMETPROGLY
23   CYSSERGLYTYRPROASNMETASPLEUASP
24   CYSLEULEUPROGLNGLUTRPVALGLNHIS
25   PHETYRGLNGLUALAALAPROALAGLNSER
26   ASPVALALALEULEUARGPHEVALASNPRO
27   ASPTHRGLYARGVALLEUPHEGLUCYSLYS
28   LEUHISLYSSERGLYTYRVALTHRVALALA
29   HISTHRGLYGLNHISASPLEUVALILEPRO
30   PROASNGLYTYRPHEARGPHEASPSERTRP
31   VALASNGLNPHETYRTHRLEUALAPROMET

Samples:

Sample_1: Molecule 1, [U-13C; U-15N; U-2H], 0.15 mM; sodium phosphate buffer 20 mM

CondSet1: pH: 7.300; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
3D HN(CO)CACBSample_1isotropicCondSet1
3D HNCACBSample_1isotropicCondSet1
3D HNCOSample_1isotropicCondSet1
3D HN(CO)CASample_1isotropicCondSet1
3D HN(CA)COSample_1isotropicCondSet1
3D HNCOSample_1isotropicCondSet1
3D HNCACBSample_1isotropicCondSet1
3D HNCASample_1isotropicCondSet1
3D HN(CO)CASample_1isotropicCondSet1

Software:

CcpNmr_Analysis v2.4, CCPN - Assignment

TopSpin v3.5, Bruker - Data Processing

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 900 MHz

Related Database Links:

GenBank AB447457

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts