BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27443

Title: 13C and 15N assignment of C', CA, CB and N of quail Osteopontin at 310K   PubMed: 30407719

Deposition date: 2018-04-09 Original release date: 2018-11-16

Authors: Mateos, Borja; Pierattelli, Roberta; Konrat, Robert; Felli, Isabella

Citation: Mateos, Borja; Konrat, Robert; Pierattelli, Roberta; Felli, Isabella. "NMR characterization of long-range contacts in intrinsically disordered proteins from paramagnetic relaxation enhancement in 13C direct-detected experiments"  Chembiochem. 20, 335-339 (2019).

Assembly members:
Osteopontin, polymer, 220 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Osteopontin: MHQDHVDSQSQEHLQQTQND LASLQQTHYSSEENADVPEQ PDFPDVPSKSQETVDDDDDD DNDSNDTDESDEVFTDFPTE APVAPFNRGDNAGRGDSVAY GFRAKAHVVKASKIRKAARK LIEDDATTEDGDSQPAGLWW PKESREQNSRELPQHQSVEN DSRPKFDSREVDGGDSKASA GVDSRESQGSVPAVDASNQT LESAEDAEDRHSIENNEVTR

Data sets:
Data typeCount
13C chemical shifts678
15N chemical shifts218

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Osteopontin monomer1

Entities:

Entity 1, Osteopontin monomer 220 residues - Formula weight is not available

1   METHISGLNASPHISVALASPSERGLNSER
2   GLNGLUHISLEUGLNGLNTHRGLNASNASP
3   LEUALASERLEUGLNGLNTHRHISTYRSER
4   SERGLUGLUASNALAASPVALPROGLUGLN
5   PROASPPHEPROASPVALPROSERLYSSER
6   GLNGLUTHRVALASPASPASPASPASPASP
7   ASPASNASPSERASNASPTHRASPGLUSER
8   ASPGLUVALPHETHRASPPHEPROTHRGLU
9   ALAPROVALALAPROPHEASNARGGLYASP
10   ASNALAGLYARGGLYASPSERVALALATYR
11   GLYPHEARGALALYSALAHISVALVALLYS
12   ALASERLYSILEARGLYSALAALAARGLYS
13   LEUILEGLUASPASPALATHRTHRGLUASP
14   GLYASPSERGLNPROALAGLYLEUTRPTRP
15   PROLYSGLUSERARGGLUGLNASNSERARG
16   GLULEUPROGLNHISGLNSERVALGLUASN
17   ASPSERARGPROLYSPHEASPSERARGGLU
18   VALASPGLYGLYASPSERLYSALASERALA
19   GLYVALASPSERARGGLUSERGLNGLYSER
20   VALPROALAVALASPALASERASNGLNTHR
21   LEUGLUSERALAGLUASPALAGLUASPARG
22   HISSERILEGLUASNASNGLUVALTHRARG

Samples:

sample_1: Osteopontin, [U-99% 13C; U-99% 15N], 2 mM

sample_conditions_1: ionic strength: 0.14 M; pH: 6.5; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D CONsample_1isotropicsample_conditions_1
3D (H)CBCACONsample_1isotropicsample_conditions_1
3D (H)CBCANCOsample_1isotropicsample_conditions_1
3D (H)COCONsample_1isotropicsample_conditions_1
3D (H)CCCONsample_1isotropicsample_conditions_1

Software:

XEASY, Keller and Wuthrich - chemical shift assignment, peak picking

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker NEO 700 MHz