BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27415

Title: Chemical shift assignments for TRAP, a MMP20 proteolysis product of murine amelogenin.   PubMed: 29960917

Authors: Buchko, Garry

Citation: Buchko, Garry; Jayasinha Arachchige, Rajith; Tao, Jinhui; Tarasevich, Barbara; Shaw, Wendy. "Identification of major matrix metalloproteinase-20 proteolytic processing products of murine amelogenin and tyrosine-rich amelogenin peptide using a nuclear magnetic resonance spectroscopy based method"  Arch. Oral Biol. 93, 187-194 (2018).

Assembly members:
TRAP, polymer, 49 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
TRAP: GPGSMPLPPHPGSPGYINLS YEVLTPLKWYQSMIRQPYPS YGYEPMGGW

Data sets:
Data typeCount
13C chemical shifts162
15N chemical shifts42
1H chemical shifts150

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TRAP1

Entities:

Entity 1, TRAP 49 residues - Formula weight is not available

1   GLYPROGLYSERMETPROLEUPROPROHIS
2   PROGLYSERPROGLYTYRILEASNLEUSER
3   TYRGLUVALLEUTHRPROLEULYSTRPTYR
4   GLNSERMETILEARGGLNPROTYRPROSER
5   TYRGLYTYRGLUPROMETGLYGLYTRP

Samples:

sample_1: TRAP, [U-99% 13C; U-99% 15N], 0.5 mM; acetic acid 2%

sample_conditions_1: ionic strength: 0 M; pH: 2.8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

Felix v2007, Accelrys Software Inc. - processing

SPARKY v1.4, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 750 MHz
  • Agilent VXRS 600 MHz
  • Agilent VXRS 750 MHz

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