BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27413

Title: 1H, 15N, 13C backbone resonance assignment of human alkbh5   PubMed: 29858729

Deposition date: 2018-03-02 Original release date: 2018-06-20

Authors: Purslow, Jeffrey; Venditti, Vincenzo

Citation: Purslow, Jeffrey; Venditti, Vincenzo. "1H, 15N, 13C backbone resonance assignment of human Alkbh5"  Biomol. NMR Assign. 12, 297-301 (2018).

Assembly members:
Alkbh5_Residues_66-292, polymer, 230 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Alkbh5_Residues_66-292: SHMPERSDYEEQQLQKEEEA RKVKSGIRQMRLFSQDECAK IEARIDEVVSRAEKGLYNEH TVDRAPLRNKYFFGEGYTYG AQLQKRGPGQERLYPPGDVD EIPEWVHQLVIQKLVEHRVI PEGFVNSAVINDYQPGGCIV SHVDPIHIFERPIVSVSFFS DSALCFGCKFQFKPIRVSEP VLSLPVRRGSVTVLSGYAAD EITHCIRPQDIKERRAVIIL RKTRLDAPRL

Data sets:
Data typeCount
13C chemical shifts609
15N chemical shifts195
1H chemical shifts195

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Alkbh5 66-292, chain 11
2Alkbh5 66-292, chain 21

Entities:

Entity 1, Alkbh5 66-292, chain 1 230 residues - Formula weight is not available

1   SERHISMETPROGLUARGSERASPTYRGLU
2   GLUGLNGLNLEUGLNLYSGLUGLUGLUALA
3   ARGLYSVALLYSSERGLYILEARGGLNMET
4   ARGLEUPHESERGLNASPGLUCYSALALYS
5   ILEGLUALAARGILEASPGLUVALVALSER
6   ARGALAGLULYSGLYLEUTYRASNGLUHIS
7   THRVALASPARGALAPROLEUARGASNLYS
8   TYRPHEPHEGLYGLUGLYTYRTHRTYRGLY
9   ALAGLNLEUGLNLYSARGGLYPROGLYGLN
10   GLUARGLEUTYRPROPROGLYASPVALASP
11   GLUILEPROGLUTRPVALHISGLNLEUVAL
12   ILEGLNLYSLEUVALGLUHISARGVALILE
13   PROGLUGLYPHEVALASNSERALAVALILE
14   ASNASPTYRGLNPROGLYGLYCYSILEVAL
15   SERHISVALASPPROILEHISILEPHEGLU
16   ARGPROILEVALSERVALSERPHEPHESER
17   ASPSERALALEUCYSPHEGLYCYSLYSPHE
18   GLNPHELYSPROILEARGVALSERGLUPRO
19   VALLEUSERLEUPROVALARGARGGLYSER
20   VALTHRVALLEUSERGLYTYRALAALAASP
21   GLUILETHRHISCYSILEARGPROGLNASP
22   ILELYSGLUARGARGALAVALILEILELEU
23   ARGLYSTHRARGLEUASPALAPROARGLEU

Samples:

sample_1: Alkbh5 Residues 66-292, [U-13C; U-15N; U-2H], 0.45 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance II 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts