BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27352

Title: Backbone 1H, 13C, and 15N assignments of the extracellular region of human Fc receptor IIIb   PubMed: 29453714

Deposition date: 2018-01-04 Original release date: 2018-02-20

Authors: Yogo, Rina; Yanaka, Saeko; Kato, Koichi

Citation: Yogo, Rina; Yanaka, Saeko; Kato, Koichi. "Backbone 1H, 13C, and 15N assignments of the extracellular region of human Fcgammaa receptor IIIb"  Biomol. NMR Assignments 12, 201-204 (2018).

Assembly members:
Fc_receptor_IIIb, polymer, 187 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Fc_receptor_IIIb: MARTEDLPKAVVFLEPQWYS VLEKDSVTLKCQGAYSPEDN STQWFHNESLISSQASSYFI DAATVNDSGEYRCQTNLSTL SDPVQLEVHIGWLLLQAPRW VFKEEDPIHLRCHSWKNTAL HKVTYLQNGKDRKYFHHNSD FHIPKATLKDSGSYFCRGLV GSKNVSSETVNITITQAAAL EHHHHHH

Data sets:
Data typeCount
13C chemical shifts501
15N chemical shifts167
1H chemical shifts167

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Fc receptor IIIb1

Entities:

Entity 1, Fc receptor IIIb 187 residues - Formula weight is not available

1   METALAARGTHRGLUASPLEUPROLYSALA
2   VALVALPHELEUGLUPROGLNTRPTYRSER
3   VALLEUGLULYSASPSERVALTHRLEULYS
4   CYSGLNGLYALATYRSERPROGLUASPASN
5   SERTHRGLNTRPPHEHISASNGLUSERLEU
6   ILESERSERGLNALASERSERTYRPHEILE
7   ASPALAALATHRVALASNASPSERGLYGLU
8   TYRARGCYSGLNTHRASNLEUSERTHRLEU
9   SERASPPROVALGLNLEUGLUVALHISILE
10   GLYTRPLEULEULEUGLNALAPROARGTRP
11   VALPHELYSGLUGLUASPPROILEHISLEU
12   ARGCYSHISSERTRPLYSASNTHRALALEU
13   HISLYSVALTHRTYRLEUGLNASNGLYLYS
14   ASPARGLYSTYRPHEHISHISASNSERASP
15   PHEHISILEPROLYSALATHRLEULYSASP
16   SERGLYSERTYRPHECYSARGGLYLEUVAL
17   GLYSERLYSASNVALSERSERGLUTHRVAL
18   ASNILETHRILETHRGLNALAALAALALEU
19   GLUHISHISHISHISHISHIS

Samples:

sample_1: Fc receptor IIIb, [U-100% 13C; U-100% 15N] partially deuterated, 0.2 mM; sodium chloride 50 mM; sodium phosphate 5 mM

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N trHSQCsample_1isotropicsample_conditions_1
3D trCBCA(CO)NHsample_1isotropicsample_conditions_1
3D trHNCACBsample_1isotropicsample_conditions_1
3D trHN(CO)CAsample_1isotropicsample_conditions_1
3D trHNCAsample_1isotropicsample_conditions_1
3D trHNCOsample_1isotropicsample_conditions_1
3D trHCACOsample_1isotropicsample_conditions_1
3D trHNCANNHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 950 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts