BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27339

Title: Chemical shifts of UBQLN2 residues 450-624   PubMed: 29526694

Deposition date: 2017-12-11 Original release date: 2018-03-16

Authors: Castaneda, Carlos

Citation: Dao, Thuy; Kolaitis, Regina-Maria; Kim, Hong Joo; O'Donovan, Kevin; Martyniak, Brian; Colicino, Erica; Hehnly, Heidi; Taylor, J Paul; Castaneda, Carlos. "Ubiquitin Modulates Liquid-Liquid Phase Separation of UBQLN2 via Disruption of Multivalent Interactions"  Mol. Cell S1097, 30102-30103 (2018).

Assembly members:
UBQLN2_450-624, polymer, 177 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
UBQLN2_450-624: MRAMQALMQIQQGLQTLATE APGLIPSFTPGVGVGVLGTA IGPVGPVTPIGPIGPIVPFT PIGPIGPIGPTGPAAPPGST GSGGPTGPTVSSAAPSETTS PTSESGPNQQFIQQMVQALA GANAPQLPNPEVRFQQQLEQ LNAMGFLNREANLQALIATG GDINAAIERLLGSQPSW

Data sets:
Data typeCount
13C chemical shifts435
15N chemical shifts147
1H chemical shifts132

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UBQLN21

Entities:

Entity 1, UBQLN2 177 residues - Formula weight is not available

Second residue R is residue 450 of human UBQLN2. Additional C-terminal residue W for prot concentration measurements.

1   METARGALAMETGLNALALEUMETGLNILE
2   GLNGLNGLYLEUGLNTHRLEUALATHRGLU
3   ALAPROGLYLEUILEPROSERPHETHRPRO
4   GLYVALGLYVALGLYVALLEUGLYTHRALA
5   ILEGLYPROVALGLYPROVALTHRPROILE
6   GLYPROILEGLYPROILEVALPROPHETHR
7   PROILEGLYPROILEGLYPROILEGLYPRO
8   THRGLYPROALAALAPROPROGLYSERTHR
9   GLYSERGLYGLYPROTHRGLYPROTHRVAL
10   SERSERALAALAPROSERGLUTHRTHRSER
11   PROTHRSERGLUSERGLYPROASNGLNGLN
12   PHEILEGLNGLNMETVALGLNALALEUALA
13   GLYALAASNALAPROGLNLEUPROASNPRO
14   GLUVALARGPHEGLNGLNGLNLEUGLUGLN
15   LEUASNALAMETGLYPHELEUASNARGGLU
16   ALAASNLEUGLNALALEUILEALATHRGLY
17   GLYASPILEASNALAALAILEGLUARGLEU
18   LEUGLYSERGLNPROSERTRP

Samples:

13C_15N_450C: UBQLN2 450-624, [U-99% 13C; U-99% 15N], 200 uM; Na Phosphate 20 mM; EDTA 0.1 mM

buffer_conditions: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
3D HNCO13C_15N_450Cisotropicbuffer_conditions
3D HNCACB13C_15N_450Cisotropicbuffer_conditions
3D CBCA(CO)NH13C_15N_450Cisotropicbuffer_conditions
3D HNCA13C_15N_450Cisotropicbuffer_conditions
3D HN(CA)CO13C_15N_450Cisotropicbuffer_conditions
2D 1H-15N HSQC13C_15N_450Cisotropicbuffer_conditions
2D N-CO (C-detect)13C_15N_450Cisotropicbuffer_conditions
3D HACANCACON (C-detect)13C_15N_450Cisotropicbuffer_conditions
3D HACANCANCO (C-detect)13C_15N_450Cisotropicbuffer_conditions

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPNMR, CCPN - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts