BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27306

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for FASP peptide of mPER2   PubMed: 29784789

Authors: Partch, Carrie; Hunt, Sabrina; Narasimamurthy, Rajesh; Lu, Yining; Fustin, Jean-Michel; Okamura, Hitoshi; Kim, Jae Kyoung; Forger, Daniel; Virshup, David

Citation: Narasimamurthy, Rajesh; Hunt, Sabrina; Lu, Yining; Fustin, Jean-Michel; Okamura, Hitoshi; Partch, Carrie; Forger, Daniel; Kim, Jae Kyoung; Virshup, David. "CK1delta/epsilon protein kinase primes the PER2 circadian phosphoswitch."  Proc. Natl. Acad. Sci. U.S.A. 115, 5986-5991 (2018).

Assembly members:
PER2_FASP, polymer, 37 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PER2_FASP: GAMDPEFWRKKKTEVSAHLS SLTLPGKAESVVSLTSQ

Data sets:
Data typeCount
13C chemical shifts100
15N chemical shifts31
1H chemical shifts31

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1mPER2 FASP1

Entities:

Entity 1, mPER2 FASP 37 residues - Formula weight is not available

Residues 1-12 represent non-native sequence. Residues 13-37 map to mPER2 residues 642-666.

1   GLYALAMETASPPROGLUPHETRPARGLYS
2   LYSLYSTHRGLUVALSERALAHISLEUSER
3   SERLEUTHRLEUPROGLYLYSALAGLUSER
4   VALVALSERLEUTHRSERGLN

Samples:

sample_1: PER2 FASP, [U-13C; U-15N], 0.4 mM; MOPS 25 mM; sodium chloride 50 mM; EDTA 1 mM; Pierce protease inhibitor tablet 1 tablet/50mL; D2O, [U-2H], 10%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNMR_Analysis v2.4.2, CCPN - chemical shift assignment, data analysis, peak picking

hmsIST, Hyberts, Takeuchi, Wagner - data reconstruction

NMR spectrometers:

  • Varian INOVA 600 MHz

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