BMRB Entry 27304

Title:
Backbone 1H and 15N Chemical Shift Assignments for HOLO-BAMB5917
Deposition date:
2017-11-07
Original release date:
2019-09-09
Authors:
Kosol, Simone; Gallo, Angelo; Griffiths, Daniel; Valentic, Timothy; Masschelein, Joleen; Jenner, Matthew; de los Santos, Emmanuel; Rea, Dean; Fulop, Vilmos; Tsai, Shiou-Chuan; Challis, Gregory; Lewandowski, Jozef
Citation:

Citation: Kosol, Simone; Gallo, Angelo; Griffiths, Daniel; Valentic, Timothy; Masschelein, Joleen; Jenner, Matthew; de Los Santos, Emmanuel; Manzi, Lucio; Sydor, Paulina; Rea, Dean; Zhou, Shanshan; Fulop, Vilmos; Oldham, Neil; Tsai, Shiou-Chuan; Challis, Gregory; Lewandowski, Jozef. "Structural basis for chain release from the enacyloxin polyketide synthase"  Nat. Chem. 11, 913-923 (2019).
PubMed: 31548674

Assembly members:

Assembly members:
BAMB5917_PCP17, polymer, 107 residues, 11682 Da.
entity_PNS, non-polymer, 358.348 Da.

Natural source:

Natural source:   Common Name: Burkholderia ambifaria   Taxonomy ID: 152480   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Burkholderia ambifaria

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET151/D-TOPO

Data sets:
Data typeCount
15N chemical shifts93
1H chemical shifts93

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BAMB59171
2PP-ARM2

Entities:

Entity 1, BAMB5917 107 residues - 11682 Da.

GIDPFT TEV cleavage site

1   GLYILEASPPROPHETHRGLYALAALAALA
2   GLYVALSERALAALAGLYILEGLUPROASP
3   LEUTHRALAILETRPGLNALALEUPHEALA
4   LEUPROALAVALGLYARGHISGLNASPPHE
5   PHEALALEUGLYGLYASPSERGLNLEUGLY
6   LEUARGMETLEUALAGLNLEUARGGLUARG
7   HISGLYVALASPLEUPROLEUARGCYSLEU
8   TYRGLUALAPROTHRVALALAARGLEUALA
9   GLUTHRILEVALARGLEUALAALAPROALA
10   PROSERGLYASPGLNASPASPALASERGLU
11   TYRGLUGLUGLYVALILEARG

Entity 2, PP-ARM - C11 H23 N2 O7 P S - 358.348 Da.

1   PNS

Samples:

sample_1: BAMB5917_PCP17, [U-100% 15N], 0.5 mM; PP-ARM 0.5 mM; D2O, [U-2H], 10%; H2O 90%; DSS 1%

sample_conditions_1: ionic strength: 150 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

UniProt Q0B311
AlphaFold Q0B311

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks