BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27258

Title: Hydrogen exchange rate of human galectin-8 CTD in apo and lactose-bound form   PubMed: 28813004

Authors: Chien, Chih-Ta Henry; Hsu, Shang-Te Danny

Citation: Chien, Chih-Ta Henry; Ho, Meng-Ru; Lin, Chung-Hung; Hsu, Shang-Te Danny. "Lactose Binding Induces Opposing Dynamics Changes in Human Galectins Revealed by NMR-Based Hydrogen-Deuterium Exchange."  Molecules 22, 1357-. (2017).

Assembly members:
galectin-8_CTD, polymer, 135 residues, Formula weight is not available
BETA-LACTOSE, non-polymer, 342.296 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
galectin-8_CTD: MRLPFAARLNTPMGPGRTVV VKGEVNANAKSFNVDLLAGK SKDIALHLNPRLNIKAFVRN SFLQESWGEEERNITSFPFS PGMYFEMIIYCDVREFKVAV NGVHSLEYKHRFKELSSIDT LEINGDIHLLEVRSW

Data sets:
Data typeCount
H exchange rates135

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1galectin-8 CTD in apo form1

Entities:

Entity 1, galectin-8 CTD in apo form 135 residues - Formula weight is not available

1   METARGLEUPROPHEALAALAARGLEUASN
2   THRPROMETGLYPROGLYARGTHRVALVAL
3   VALLYSGLYGLUVALASNALAASNALALYS
4   SERPHEASNVALASPLEULEUALAGLYLYS
5   SERLYSASPILEALALEUHISLEUASNPRO
6   ARGLEUASNILELYSALAPHEVALARGASN
7   SERPHELEUGLNGLUSERTRPGLYGLUGLU
8   GLUARGASNILETHRSERPHEPROPHESER
9   PROGLYMETTYRPHEGLUMETILEILETYR
10   CYSASPVALARGGLUPHELYSVALALAVAL
11   ASNGLYVALHISSERLEUGLUTYRLYSHIS
12   ARGPHELYSGLULEUSERSERILEASPTHR
13   LEUGLUILEASNGLYASPILEHISLEULEU
14   GLUVALARGSERTRP

Samples:

sample_1: galectin-8 CTD, [U-100% 15N], 200 uM

sample_2: galectin-8 CTD, [U-100% 15N], 200 uM; Lactose 31.3 mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

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