BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27257

Title: Hydrogen exchange rate of human galectin-8 NTD in apo and lactose-bound form   PubMed: 28813004

Authors: Chien, Chih-Ta Henry; Hsu, Shang-Te Danny

Citation: Chien, Chih-Ta Henry; Ho, Meng-Ru; Lin, Chung-Hung; Hsu, Shang-Te Danny. "Lactose Binding Induces Opposing Dynamics Changes in Human Galectins Revealed by NMR-Based Hydrogen-Deuterium Exchange."  Molecules 22, 1357-. (2017).

Assembly members:
galectin-8_NTD, polymer, 155 residues, Formula weight is not available
BETA-LACTOSE, non-polymer, 342.296 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
galectin-8_NTD: MMLSLNNLQNIIYNPVIPFV GTIPDQLDPGTLIVIRGHVP SDADRFQVDLQNGSSMKPRA DVAFHFNPRFKRAGCIVCNT LINEKWGREEITYDTPFKRE KSFEIVIMVLKDKFQVAVNG KHTLLYGHRIGPEKIDTLGI YGKVNIHSIGFSFSS

Data sets:
Data typeCount
H exchange rates138

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1galectin-8 NTD in apo form1

Entities:

Entity 1, galectin-8 NTD in apo form 155 residues - Formula weight is not available

1   METMETLEUSERLEUASNASNLEUGLNASN
2   ILEILETYRASNPROVALILEPROPHEVAL
3   GLYTHRILEPROASPGLNLEUASPPROGLY
4   THRLEUILEVALILEARGGLYHISVALPRO
5   SERASPALAASPARGPHEGLNVALASPLEU
6   GLNASNGLYSERSERMETLYSPROARGALA
7   ASPVALALAPHEHISPHEASNPROARGPHE
8   LYSARGALAGLYCYSILEVALCYSASNTHR
9   LEUILEASNGLULYSTRPGLYARGGLUGLU
10   ILETHRTYRASPTHRPROPHELYSARGGLU
11   LYSSERPHEGLUILEVALILEMETVALLEU
12   LYSASPLYSPHEGLNVALALAVALASNGLY
13   LYSHISTHRLEULEUTYRGLYHISARGILE
14   GLYPROGLULYSILEASPTHRLEUGLYILE
15   TYRGLYLYSVALASNILEHISSERILEGLY
16   PHESERPHESERSER

Samples:

sample_1: galectin-8 NTD, [U-100% 15N], 200 uM

sample_2: galectin-8 NTD, [U-100% 15N], 200 uM; Lactose 2.4 mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links: