BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27244

Title: Chemical shifts assignments of Escherichia coli aquaporin (AqpZ) by solid-state NMR

Deposition date: 2017-09-06 Original release date: 2018-10-02

Authors: Xie, Hua yong; Zhao, Yong xiang; yang, Jun

Citation: Xie, Hua yong; Zhao, Yong xiang. "Chemical shifts assignments of Escherichia coli aquaporin (AqpZ) by solid-state NMR"  Angew. Chem. Int. Ed. Engl. ., .-..

Assembly members:
AqpZ, polymer, 243 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
AqpZ: MGSSHHHHHHEFMFRKLAAE CFGTFWLVFGGCGSAVLAAG FPELGIGFAGVALAFGLTVL TMAFAVGHISGGHFNPAVTI GLWAGGRFPAKEVVGYVIAQ VVGGIVAAALLYLIASGKTG FDAAASGFASNGYGEHSPGG YSMLSALVVELVLSAGFLLV IHGATDKFAPAGFAPIAIGL ALTLIHLISIPVTNTSVNPA RSTAVAIFQGGWALEQLWFF WVVPIVGGIIGGLIYRTLLE KRD

Data sets:
Data typeCount
13C chemical shifts840
15N chemical shifts220

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AqpZ1

Entities:

Entity 1, AqpZ 243 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   GLUPHEMETPHEARGLYSLEUALAALAGLU
3   CYSPHEGLYTHRPHETRPLEUVALPHEGLY
4   GLYCYSGLYSERALAVALLEUALAALAGLY
5   PHEPROGLULEUGLYILEGLYPHEALAGLY
6   VALALALEUALAPHEGLYLEUTHRVALLEU
7   THRMETALAPHEALAVALGLYHISILESER
8   GLYGLYHISPHEASNPROALAVALTHRILE
9   GLYLEUTRPALAGLYGLYARGPHEPROALA
10   LYSGLUVALVALGLYTYRVALILEALAGLN
11   VALVALGLYGLYILEVALALAALAALALEU
12   LEUTYRLEUILEALASERGLYLYSTHRGLY
13   PHEASPALAALAALASERGLYPHEALASER
14   ASNGLYTYRGLYGLUHISSERPROGLYGLY
15   TYRSERMETLEUSERALALEUVALVALGLU
16   LEUVALLEUSERALAGLYPHELEULEUVAL
17   ILEHISGLYALATHRASPLYSPHEALAPRO
18   ALAGLYPHEALAPROILEALAILEGLYLEU
19   ALALEUTHRLEUILEHISLEUILESERILE
20   PROVALTHRASNTHRSERVALASNPROALA
21   ARGSERTHRALAVALALAILEPHEGLNGLY
22   GLYTRPALALEUGLUGLNLEUTRPPHEPHE
23   TRPVALVALPROILEVALGLYGLYILEILE
24   GLYGLYLEUILETYRARGTHRLEULEUGLU
25   LYSARGASP

Samples:

sample_1: AqpZ, [U-13C; U-15N], 70 % w/w; POPC/POPG lipids % w/w

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 277 K

Experiments:

NameSampleSample stateSample conditions
3D NCACXsample_1isotropicsample_conditions_1
3D NCOCXsample_1isotropicsample_conditions_1
3D CONCAsample_1isotropicsample_conditions_1
3D CANCOCXsample_1isotropicsample_conditions_1
3D NCACBsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

VNMRJ v2.31A, Varian - collection

NMR spectrometers:

  • Varian Uniform NMR System 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

NCBI GenBank: CCI77899.1 GenBank: CDO13762.1