BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27232

Title: 13C chemical shifts of solid-state NMR signals of subunit c-ring of TFoF1 ATP synthase

Authors: Akutsu, Hideo; Kang, Sujin; Todokoro, Yasuto; Bak, Suyeon; Suzuki, Toshiharu; Yoshida, Masasuke; Fujiwara, Toshimichi

Citation: Akutsu, Hideo; Kang, Sujin; Todokoro, Yasuto; Suzuki, Toshiharu; Yoshida, Masasuke; Fujiwara, Toshimichi. "Direct assignment of 13C solid-state NMR signals of TFoc-ring in lipid membranes and its implication for the ring structure"  J. Biomol. NMR ., .-..

Assembly members:
FoF1_ATP_synthase_subunit_c, polymer, 72 residues, 7384 Da.

Natural source:   Common Name: thermophilic Bacillus   Taxonomy ID: 1386   Superkingdom: Bacteria   Kingdom: not available   Genus/species: thermophilic Bacillus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
FoF1_ATP_synthase_subunit_c: MHLGVLAAAIAVGLGALGAG IGNGLIVSRTIEGIARQPEL RPVLQTTMFIGVALVEALPI IGVVFSFIYLGR

Data sets:
Data typeCount
13C chemical shifts294

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ATP synthase subunit c1

Entities:

Entity 1, ATP synthase subunit c 72 residues - 7384 Da.

1   METHISLEUGLYVALLEUALAALAALAILE
2   ALAVALGLYLEUGLYALALEUGLYALAGLY
3   ILEGLYASNGLYLEUILEVALSERARGTHR
4   ILEGLUGLYILEALAARGGLNPROGLULEU
5   ARGPROVALLEUGLNTHRTHRMETPHEILE
6   GLYVALALALEUVALGLUALALEUPROILE
7   ILEGLYVALVALPHESERPHEILETYRLEU
8   GLYARG

Samples:

Rev1: FoF1 ATP synthase subunit c-ring, [U-13C], 3 mg

Rev2: FoF1 ATP synthase subunit c-ring, [U-13C], 3 mg

Rev3: FoF1 ATP synthase subunit c-ring, [U-13C], 3 mg

sample_condition_1: ionic strength: 10 mM; pH: 7.5; temperature: 248 K

Experiments:

NameSampleSample stateSample conditions
2D 13C homonuclear correlation experiments with the dipolar assisted rotational resonance (DARR)Rev1isotropicsample_condition_1
2D 13C homonuclear correlation experiments with the dipolar assisted rotational resonance (DARR)Rev2isotropicsample_condition_1
2D 13C homonuclear correlation experiments with the dipolar assisted rotational resonance (DARR)Rev3isotropicsample_condition_1
2D Cai+1-(C Ca)i correlation spectraRev1isotropicsample_condition_1
2D Cai+1-(C Ca)i correlation spectraRev2isotropicsample_condition_1
2D Cai+1-(C Ca)i correlation spectraRev3isotropicsample_condition_1

Software:

SPARKY v3.114, Goddard & Kneller (SPARKY) - chemical shift assignment

Felix vFelix2007, Felix NMR Inc. - processing

NMR spectrometers:

  • Varian Infinity-plus 600 600 MHz
  • Varian Infinity-plus 700 700 MHz

Related Database Links:

UniProt KB/Swiss Prot P00845