BMRB Entry 27192

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27192

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
NMR assignment of Rab5a (double deletion mutant) from Leishmania donovani
Deposition date:
2017-07-22
Original release date:
2019-01-10
Authors:
Maheshwari, Diva; Yadav, Rahul; Mukhopadhyay, Amitabha; Arora, Ashish
Citation:

Citation: Maheshwari, Diva; Yadav, Rahul; Rastogi, Ruchir; Jain, Anupam; Tripathi, Sarita; Mukhopadhyay, Amitabha; Arora, Ashish. "Structural and Biophysical Characterization of Rab5a from Leishmania Donovani"  Biophys. J. 115, 1217-1230 (2018).
PubMed: 30241678

Assembly members:

Assembly members:
Rab5a, polymer, 175 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Leishmania donovani   Taxonomy ID: 5661   Superkingdom: Eukaryota   Kingdom: Excavata   Genus/species: Leishmania donovani

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rab5a: MNTHPPQLMEATSAKIVMLG ESGAGKSSIALRFTRNEFLA NQETTIGAAFLSKTVMIDGR ALKYEIWDTAGLERFRSLAP IYYRGASGALVVYDITNSES LKKAQTWIKELRANADPSLI IVLVGNKKDLGSLRQVSFED GQRLAAEEQLAAFYEASAKD NNNVEQVFLDLAAKL

Data sets:
Data typeCount
13C chemical shifts632
15N chemical shifts165
1H chemical shifts1024

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rab5a1

Entities:

Entity 1, Rab5a 175 residues - Formula weight is not available

1   METASNTHRHISPROPROGLNLEUMETGLU
2   ALATHRSERALALYSILEVALMETLEUGLY
3   GLUSERGLYALAGLYLYSSERSERILEALA
4   LEUARGPHETHRARGASNGLUPHELEUALA
5   ASNGLNGLUTHRTHRILEGLYALAALAPHE
6   LEUSERLYSTHRVALMETILEASPGLYARG
7   ALALEULYSTYRGLUILETRPASPTHRALA
8   GLYLEUGLUARGPHEARGSERLEUALAPRO
9   ILETYRTYRARGGLYALASERGLYALALEU
10   VALVALTYRASPILETHRASNSERGLUSER
11   LEULYSLYSALAGLNTHRTRPILELYSGLU
12   LEUARGALAASNALAASPPROSERLEUILE
13   ILEVALLEUVALGLYASNLYSLYSASPLEU
14   GLYSERLEUARGGLNVALSERPHEGLUASP
15   GLYGLNARGLEUALAALAGLUGLUGLNLEU
16   ALAALAPHETYRGLUALASERALALYSASP
17   ASNASNASNVALGLUGLNVALPHELEUASP
18   LEUALAALALYSLEU

Samples:

sample_1: sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1%; DTT 2 mM; GTP 1 mM; Magnesium chloride 5 mM; Glycerol 3%; Rab5a

sample_2: sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1%; DTT 2 mM; GTP 1 mM; Magnesium chloride 5 mM; Glycerol 3%; Rab5a

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks