BMRB Entry 27164

Title:
3Jcgn & 3Jcgc' scalar couplings for hnRNP A1 RRM1
Deposition date:
2017-06-27
Original release date:
2022-06-23
Authors:
Diarra dit Konte, N.; Damberger, F.; Allain, F.
Citation:

Citation: Diarra dit Konte, N.; Krepl, M.; Damberger, F.; Ripin, N.; Duss, O.; Sponer, J.; Allain, F.. "Aromatic side-chain conformational switch on the surface of the RNA Recognition Motif enables RNA discrimination"  Nat. Commun. 8, 654-654 (2017).
PubMed: 28935965

Assembly members:

Assembly members:
entity_1, polymer, 118 residues, Formula weight is not available
entity_2, polymer, 7 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet28a+

Data sets:
Data typeCount
coupling constants14

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hnRNP A1 RRM11
2UUAGGCU2

Entities:

Entity 1, hnRNP A1 RRM1 118 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METALASERLYSSERGLUSERPROLYSGLU
4   PROGLUGLNLEUARGLYSLEUPHEILEGLY
5   GLYLEUSERPHEGLUTHRTHRASPGLUSER
6   LEUARGSERHISPHEGLUGLNTRPGLYTHR
7   LEUTHRASPCYSVALVALMETARGASPPRO
8   ASNTHRLYSARGSERARGGLYPHEGLYPHE
9   VALTHRTYRALATHRVALGLUGLUVALASP
10   ALAALAMETASNALAARGPROHISLYSVAL
11   ASPGLYARGVALVALGLUPROLYSARGALA
12   VALSERARGGLUASPSERGLNARG

Entity 2, UUAGGCU 7 residues - Formula weight is not available

1   UUAGGCU

Samples:

sample_1: entity_1, [U-99% 13C; U-99% 15N], 1.2 mM; H2O 90 % v/v; D2O 10 % v/v; sodium phosphate 10 mM; beta-mercaptoethanol 10 mM; DEPC 0.1 % v/v; entity_2 1.2 mM

sample_conditions_1: ionic strength: 130 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
spin-echo difference constant time 15N-HSQCsample_1isotropicsample_conditions_1
spin-echo difference constant time HN(CO)CGsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker AvanceIII 500 MHz