BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27129

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for T94T variant of human liver FABP   PubMed: 28668637

Authors: D'Onofrio, Mariapina; Barracchia, Carlo Giorgio; Bortot, Andrea; Munari, Francesca; Zanzoni, Serena; Assfalg, Michael

Citation: D'Onofrio, Mariapina; Barracchia, Carlo Giorgio; Bortot, Andrea; Munari, Francesca; Zanzoni, Serena; Assfalg, Michael. "Molecular differences between human liver fatty acid binding protein and its T94A variant in their unbound and lipid-bound states"  Biochim. Biophys. Acta 1865, 1152-1159 (2017).

Assembly members:
hL-FABP, polymer, 135 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
hL-FABP: MRGSMSFSGKYQLQSQENFE AFMKAIGLPEELIQKGKDIK GVSEIVQNGKHFKFTITAGS KVIQNEFTVGEECELETMTG EKVKTVVQLEGDNKLVTTFK NIKSVTELNGDIITNTMTLG DIVFKRISKRILVPR

Data sets:
Data typeCount
13C chemical shifts239
15N chemical shifts126
1H chemical shifts126
T1 relaxation values109
T2 relaxation values104

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1hL-FABP1

Entities:

Entity 1, hL-FABP 135 residues - Formula weight is not available

First and last four residues are added for cloning and tag purposes

1   METARGGLYSERMETSERPHESERGLYLYS
2   TYRGLNLEUGLNSERGLNGLUASNPHEGLU
3   ALAPHEMETLYSALAILEGLYLEUPROGLU
4   GLULEUILEGLNLYSGLYLYSASPILELYS
5   GLYVALSERGLUILEVALGLNASNGLYLYS
6   HISPHELYSPHETHRILETHRALAGLYSER
7   LYSVALILEGLNASNGLUPHETHRVALGLY
8   GLUGLUCYSGLULEUGLUTHRMETTHRGLY
9   GLULYSVALLYSTHRVALVALGLNLEUGLU
10   GLYASPASNLYSLEUVALTHRTHRPHELYS
11   ASNILELYSSERVALTHRGLULEUASNGLY
12   ASPILEILETHRASNTHRMETTHRLEUGLY
13   ASPILEVALPHELYSARGILESERLYSARG
14   ILELEUVALPROARG

Samples:

sample_1: human liver fatty acid binding protein, [U-100% 13C; U-100% 15N], 0.5 mM; phosphate buffer 10 mM

sample_2: human liver fatty acid binding protein, [U-100% 15N], 0.5 mM; phosphate buffer 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
standard Heteronuclear 15N T1sample_2isotropicsample_conditions_1
standard Heteronuclear 15N T2sample_2isotropicsample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links: