BMRB Entry 27103

Title:
Backbone assignment for Met66 prodomain of BDNF
Deposition date:
2017-05-09
Original release date:
2021-07-22
Authors:
Wang, Jing; Bracken, Clay; Bains, Henrietta; Anastasia, Agustin
Citation:

Citation: Wang, Jing; Bains, Henrietta; Anastasia, Agustin; Bracken, Clay. "NMR backbone resonance assignments of the prodomain variants of BDNF in the urea denatured state"  Biomol. NMR Assign. 12, 43-45 (2018).
PubMed: 28933046

Assembly members:

Assembly members:
Met66_BDNF_prodomain, polymer, 112 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Data sets:
Data typeCount
13C chemical shifts311
15N chemical shifts103
1H chemical shifts103

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Met66 BDNF prodomain1

Entities:

Entity 1, Met66 BDNF prodomain 112 residues - Formula weight is not available

1   SERMETALAPROMETLYSGLUALAASNILE
2   ARGGLYGLNGLYGLYLEUALATYRPROGLY
3   VALARGTHRHISGLYTHRLEUGLUSERVAL
4   ASNGLYPROLYSALAGLYSERARGGLYLEU
5   THRSERLEUALAASPTHRPHEGLUHISMET
6   ILEGLUGLULEULEUASPGLUASPGLNLYS
7   VALARGPROASNGLUGLUASNASNLYSASP
8   ALAASPLEUTYRTHRSERARGVALMETLEU
9   SERSERGLNVALPROLEUGLUPROPROLEU
10   LEUPHELEULEUGLUGLUTYRLYSASNTYR
11   LEUASPALAALAASNMETSERMETARGVAL
12   ARGARG

Samples:

sample_1: Met66 BDNF prodomain, [U-95% 13C; U-95% 15N], 0.3 mM; urea 2 M; sodium chloride 100 mM; TRIS 10 mM; glycine 10 mM; PIPES 10 mM; DSS 0.1 mM; D2O 7%; H2O 93%; EDTA 0.5 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.2; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks