BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27064

Title: Solution structure of the IgI domain of CD147   PubMed: 28822092

Deposition date: 2017-04-05 Original release date: 2017-12-14

Authors: Jin, Shujuan; Xia, Bin

Citation: Jin, Shujuan; Ding, Pengfei; Chu, Pengxiang; Li, Hongwei; Sun, Jianbo; Liang, Dehai; Song, Fei; Xia, Bin. "Zn(II) can mediate self-association of the extracellular C-terminal domain of CD147."  Protein Cell 9, 310-315 (2018).

Assembly members:
CD147, polymer, 107 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CD147: IQLHGPPRVKAVKSSEHINE GETAMLVCKSESVPPVTDWA WYKITDSEDKALMNGSESRF FVSSSQGRSELHIENLNMEA DPGQYRCNGTSSKGSDQAII TLRVRSH

Data sets:
Data typeCount
13C chemical shifts430
15N chemical shifts113
1H chemical shifts698

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CD1471

Entities:

Entity 1, CD147 107 residues - Formula weight is not available

1   ILEGLNLEUHISGLYPROPROARGVALLYS
2   ALAVALLYSSERSERGLUHISILEASNGLU
3   GLYGLUTHRALAMETLEUVALCYSLYSSER
4   GLUSERVALPROPROVALTHRASPTRPALA
5   TRPTYRLYSILETHRASPSERGLUASPLYS
6   ALALEUMETASNGLYSERGLUSERARGPHE
7   PHEVALSERSERSERGLNGLYARGSERGLU
8   LEUHISILEGLUASNLEUASNMETGLUALA
9   ASPPROGLYGLNTYRARGCYSASNGLYTHR
10   SERSERLYSGLYSERASPGLNALAILEILE
11   THRLEUARGVALARGSERHIS

Samples:

sample_1: CD147, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 50 mM; sodium chloride 50 mM; EDTA 5 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts