BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27045

Title: Solid-state NMR chemical shifts of amyloid-like fibrils formed by huntingtin exon 1 with a 44-residue polyQ domain.

Authors: Hoop, Cody; Kar, Karunakar; Wetzel, Ronald; van der Wel, Patrick; Lin, Hsiang-Kai; Poirier, Michelle; Boatz, Jennifer

Citation: Hoop, Cody; Lin, Hsiang-Kai; Kar, Karunakar; Hou, Zhipeng; Poirier, Michelle; Wetzel, Ronald; van der Wel, Patrick; Magyarfalvi, Gabor; Lamley, Jonathan; Boatz, Jennifer; Mandal, Abhishek; Lewandowski, Jozef. "Polyglutamine amyloid core boundaries and flanking domain dynamics in huntingtin fragment fibrils determined by solid-state NMR"  . ., .-..

Assembly members:
huntingtin_exon1_Q44, polymer, 120 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
huntingtin_exon1_Q44: MATLEKLMKAFESLKSFQQQ QQQQQQQQQQQQQQQQQQQQ QQQQQQQQQQQQQQQQQQQQ QPPPPPPPPPPPQLPQPPPQ AQPLLPQPQPPPPPPPPPPG PAVAEEPLHRPSGSHHHHHH

Data typeCount
13C chemical shifts130
15N chemical shifts8

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HttExon1, Chain A1
2HttExon1, Chain B1

Entities:

Entity 1, HttExon1, Chain A 120 residues - Formula weight is not available

1   METALATHRLEUGLULYSLEUMETLYSALA
2   PHEGLUSERLEULYSSERPHEGLNGLNGLN
3   GLNGLNGLNGLNGLNGLNGLNGLNGLNGLN
4   GLNGLNGLNGLNGLNGLNGLNGLNGLNGLN
5   GLNGLNGLNGLNGLNGLNGLNGLNGLNGLN
6   GLNGLNGLNGLNGLNGLNGLNGLNGLNGLN
7   GLNPROPROPROPROPROPROPROPROPRO
8   PROPROGLNLEUPROGLNPROPROPROGLN
9   ALAGLNPROLEULEUPROGLNPROGLNPRO
10   PROPROPROPROPROPROPROPROPROGLY
11   PROALAVALALAGLUGLUPROLEUHISARG
12   PROSERGLYSERHISHISHISHISHISHIS

Samples:

sample_1: huntingtin_exon1_Q44, [U-99% 13C; U-99% 15N], 5 mg

275_K: temperature: 275 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-13C DARRsample_1solid275_K
2D 13C-13C DARRsample_1solid275_K
2D 13C-13C INEPT/TOBSYsample_1solid275_K
2D 13C-13C PDSDsample_1solid275_K
1H-13C DIPSHIFTsample_1solid275_K

Software:

SPARKY, Goddard - chemical shift assignment

TOPSPIN, Bruker Biospin, CCPN - chemical shift assignment, collection

CcpNMR_Analysis, Bruker Biospin, CCPN - chemical shift assignment, collection

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 750 MHz

Related Database Links: